Difference between revisions of "Team:UNOTT/Results"

Line 66: Line 66:
 
}
 
}
  
.dropdown {
+
.dropdown1 {
 
   float: left;
 
   float: left;
 
   width: 100%;
 
   width: 100%;
Line 73: Line 73:
 
}
 
}
  
.dropdown input[type=checkbox] {
+
.dropdown1 input[type=checkbox] {
 
   position: absolute;
 
   position: absolute;
 
   top: 0;
 
   top: 0;
Line 83: Line 83:
 
}
 
}
  
.dropdown label {
+
.dropdown1 label {
 
   cursor: pointer;
 
   cursor: pointer;
 
   display: inline-block;
 
   display: inline-block;
Line 94: Line 94:
 
}
 
}
  
.dropdown label:before {
+
.dropdown1 label:before {
 
   content: "";
 
   content: "";
 
   position: absolute;
 
   position: absolute;
Line 105: Line 105:
 
}
 
}
  
.dropdown input[type=checkbox]:checked ~ label:before {
+
.dropdown1 input[type=checkbox]:checked ~ label:before {
 
   border-left: 8px solid transparent;
 
   border-left: 8px solid transparent;
 
   border-top: 8px solid #F5634A;
 
   border-top: 8px solid #F5634A;
Line 112: Line 112:
 
}
 
}
  
.dropdown .text {
+
.dropdown1 .text {
 
   max-height: 0;
 
   max-height: 0;
 
   -webkit-transition: max-height 1s;  
 
   -webkit-transition: max-height 1s;  
Line 190: Line 190:
  
 
<div class="wrapper">
 
<div class="wrapper">
   <div class="dropdown">
+
   <div class="dropdown1">
 
   <input type="checkbox" id="question-1">
 
   <input type="checkbox" id="question-1">
 
   <label for="question-1">Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor?</label>
 
   <label for="question-1">Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor?</label>
Line 197: Line 197:
 
     </div>
 
     </div>
 
   </div>
 
   </div>
   <div class="dropdown">
+
   <div class="dropdown1">
 
   <input type="checkbox" id="question-2">
 
   <input type="checkbox" id="question-2">
 
   <label for="question-2">Lorem ipsum dolor sit amet, consectetur adipisicing?</label>
 
   <label for="question-2">Lorem ipsum dolor sit amet, consectetur adipisicing?</label>
Line 204: Line 204:
 
     </div>
 
     </div>
 
   </div>
 
   </div>
   <div class="dropdown">
+
   <div class="dropdown1">
 
   <input type="checkbox" id="question-3">
 
   <input type="checkbox" id="question-3">
 
   <label for="question-3">Magna aliqua ut enim ad minim?</label>
 
   <label for="question-3">Magna aliqua ut enim ad minim?</label>
Line 211: Line 211:
 
     </div>
 
     </div>
 
   </div>
 
   </div>
   <div class="dropdown">
+
   <div class="dropdown1">
 
   <input type="checkbox" id="question-4">
 
   <input type="checkbox" id="question-4">
 
   <label for="question-4">Lorem ipsum dolor sit amet, consectetur adipisicing?</label>
 
   <label for="question-4">Lorem ipsum dolor sit amet, consectetur adipisicing?</label>

Revision as of 20:02, 29 October 2017





RESULTS:

STEP 1: Create guideRNA Plasmid

STEP 2: Create Reporter Plasmid

STEP 3: Create Promoter Library

STEP 4: Random Ligations

STEP 5: Freeze drying & Revivial

STEP 6: CRISPRi & guideRNA efficiency

This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.