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Revision as of 20:03, 29 October 2017
RESULTS:
STEP 1: Create guideRNA Plasmid
STEP 2: Create Reporter Plasmid
STEP 3: Create Promoter Library
STEP 4: Random Ligations
STEP 5: Freeze drying & Revivial
STEP 6: CRISPRi & guideRNA efficiency
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.
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