Difference between revisions of "Team:Stony Brook/InterLab"

Line 114: Line 114:
 
<p>Then we took the fluorescence and absorbance readings on our plate reader for the time intervals, 0 hours, 2 hours, 4 hours, and 6 hours. The raw readings are listed in the tables below.  
 
<p>Then we took the fluorescence and absorbance readings on our plate reader for the time intervals, 0 hours, 2 hours, 4 hours, and 6 hours. The raw readings are listed in the tables below.  
  
<img src="https://static.igem.org/mediawiki/2017/b/bb/T--Stony_Brook--interlab3-3.png" style="text-align: center;width:250px;height:250px;"/>
+
<img src="https://static.igem.org/mediawiki/2017/8/80/T--Stony_Brook--interlab3-3.png" style="text-align: center;width:250px;height:250px;"/>
  
 
<p>If we compare the absorbance readings for Colony 1 and Relicate 1 for all transformations over the course of the 2-hour increments, we see that there is a general increase in the absorbance of cells for most of the plates. As expected, we can infer the cells are growing over time, thus absorbing more light. We also notice that there are low levels of absorbance in Test Device 1 compared to the other devices and controls.</p>
 
<p>If we compare the absorbance readings for Colony 1 and Relicate 1 for all transformations over the course of the 2-hour increments, we see that there is a general increase in the absorbance of cells for most of the plates. As expected, we can infer the cells are growing over time, thus absorbing more light. We also notice that there are low levels of absorbance in Test Device 1 compared to the other devices and controls.</p>

Revision as of 00:28, 30 October 2017

Stony Brook 2017

This year, our team participated in the iGEM Fourth InterLab study. The objective of this study is to improve the measurement tools in order to obtain more reliable data within the field of synthetic biology. This year’s study aimed to improve the GFP and OD600 measurement protocol via the use of a plate reader. We were provided with 8 plasmids, including 2 controls and 6 test devices, to transform into DH5-alpha Escherichia Coli cells. We measured the fluorescence and absorbance with the Molecular Devices Microplate Reader SoftMax Pro 6.5.1.