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Revision as of 09:19, 30 October 2017
From CU we cloned cells that contained our part. Then we did miniprep hence purified the plasmid. After that we sent the parts to BGI for sequencing. The results are shown below. By subtracting 1000 bp of the template sequence aligned with our own sequence, it is found that the both matches with each other.
Base on the fact we can conclude that we have successfully cloned
Here are the results:
From the pictures below, the continuous grey line formed by rectangle boxes represents the theoretical sequence from the IDT,. Below it is the sequence of the gene we cloned. While there are flaws such as mutations along the gene we cloned (highlighted in red colour)
(Clicking on images will open the enlarged version in new tab)