Difference between revisions of "Team:Hong Kong UCCKE/Results"

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Revision as of 09:21, 30 October 2017

From CU we cloned cells that contained our part. Then we did miniprep hence purified the plasmid. After that we sent the parts to BGI for sequencing. The results are shown below. By subtracting 1000 bp of the template sequence aligned with our own sequence, it is found that the both matches with each other.

Base on the fact we can conclude that we have successfully cloned

Here are the results:

From the pictures below, the continuous grey line formed by rectangle boxes represents the theoretical sequence from the IDT,. Below it is the sequence of the gene we cloned. While there are flaws such as mutations along the gene we cloned (highlighted in red colour)

(Clicking on images will open the enlarged version in new tab)

Project 300


Image of gel electrophoresis of 300

Project 300 (1)

Project 300 (2)

Project 400


Image of gel electrophoresis of 400

Project 400 (1)

Project 400 (2)

Project 500


Image of gel electrophoresis of 500

Project 500 (1)

Project 500 (2)

Project 502


Project 502 (1)

Project 502 (2)