Difference between revisions of "Team:MSU-Michigan/IPTG Induction"

Revision as of 21:16, 30 October 2017

IPTG Induction

IPTG Induction of various strains of Shewanella Oneidnensis.

M5 Media Fluorescence

Control (M5 Media only) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.

mtrB Induction Fluorescence

Induction of mtrB gene (no GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. - Acts as a second control

mtrB_GFP Induction Fluorescence

Induction of mtrB_GFP strain (GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. This is the highest because resources are not being directed toward the mtrB pathway, which is still removed

mrtB_GFP_mtrB Induction Fluorescence

Induction of mtrB_GFP_mtrB strain (GFP, mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.

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mtrB GFP Induction Current

Induction of mtrB GFP measured using a potentiostat under anaerobic conditions in a single cell bioreactor. The sample was induced with 200uM IPTG at 1.98 hours.