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Revision as of 00:20, 31 October 2017


Logo
Overview Motivation CAR T Cells Tumor Microenvironment AND Gate Outlook Achievements References
Members Attributions Partners Contact
Main Project Modeling Applied Design Proof of Concept BioBricks Basic Part BioBrick Improvement Interlab Study
Safety Cloning HIF1A Knockdown Cell Culture Lab Book
Human Practice Integrated Human Practice Collaborations

Modeling

Formula Numeri

Finding the CARTELTM AND gate

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The hypoxia response element (HRE), cAMP response element (CRE) and CTLA4 promoter can be utilized for a more specific CAR expression. Combining these three enhancers in an AND gate to allow a specific CAR expression (Brophy et al., 2014) proved to be quite challenging. Using endogenous systems came with a drawback - a knockout or knockdown of either HIF1, VEGFR-2 or TDAG8 had to be generated.

f'(t)=a(z)-k1*f(t)

(1)

y'(t)=k2*f(t)-k3*y(t)

(2)

Applied Design

BioBricks