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+ | <h4>Introduction</h4> | ||
+ | <td><p>Used in conjunction with appropriate antibiotics to selectively plate</p> | ||
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Revision as of 00:20, 31 October 2017
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Interlab Study
We are very excited to announce we've transformed our DNA parts for the 2017 iGEM Interlab Study. Check out our Twitter page Follow @RPIiGEM for weekly updates on our project!
We will be using DNA parts from Plate 7 in the distribution kit and are looking forward to collecting our first round of data.
♦Process Flow♦
Steps with an asterisk are not required in the iGEM interlab study, rather they are control steps to preserve the DNA and ensure that if anything goes wrong, we have a stock to retransform We choose two colonies per plate so we can run biological duplicates. This can provide information on variability of compounds within a single organism (in this case, DH5a e. Coli) The iGEM plate reader protocol has a series of dilutions, so we will have to pay careful attention to those details when the time comes Form 1 can be filled out at any time, Form 2 requires spreadsheet data from plate reader, Form 3 is in regard to the culturing process |
♦Protocols♦
LB Agar Plates
Used in conjunction with appropriate antibiotics to selectively plate |