Difference between revisions of "Team:RPI Troy NY/HP/Silver"
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<h2>Philosophy</h2> | <h2>Philosophy</h2> | ||
| − | <p>Oil spills pose a sizeable threat to marine life and are classified as a major environmental disaster. Cleaning up after oil spills involves the use of dispersants. Chemical dispersants break down crude oil for easier biodegradation. This process depends on location and wind speed | + | <p>Oil spills pose a sizeable threat to marine life and are classified as a major environmental disaster. Cleaning up after oil spills involves the use of dispersants. Chemical dispersants break down crude oil for easier biodegradation. This process depends on location and wind speed in addition to the use of mechanical human-guided skimmers that manually contain and collect oil. Current containment methods include surrounding the spill using floating booms to prevent further spread. </p> |
| − | <p>Sophorolipids | + | <p>Sophorolipids (an example of biosurfactants) are natural dispersers that promote degradation of crude oil. The majority of sophorolipids are biodegradable and pose little toxicity to humans. Our team aims to create sophorolipids using the fermentation of the yeast Starmarella bombicola. We aim to improve sophorolipid production by disabling an enzyme regulating the sopholipid concentration.</p> |
</div> | </div> | ||
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<div class="column half_size"> | <div class="column half_size"> | ||
| − | <h2>Public Engagement / Dialogue</h2> | + | <h2>Public Engagement/Dialogue</h2> |
| − | <p>Through open communication with the community at large through social media as well as direct interaction | + | <p>Through open communication with the community at large through social media as well as direct interaction, we aim to share knowledge regarding biosurfactants to inform the public. A two-way dialogue between our research group and the community can support the exchange of information for mutual benefits. |
</p> | </p> | ||
</div> | </div> | ||
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<div class="column half_size"> | <div class="column half_size"> | ||
<h2>Education</h2> | <h2>Education</h2> | ||
| − | <p>Sophorolipids are carbohydrate-based, ampiphilic biosurfactants. They have been historically employed in oil spills to help mitigate their damaging environmental effect by accelerating crude oil degradation. Sophorolipids are the fermentation byproducts of the Starmarella | + | <p>Sophorolipids are carbohydrate-based, ampiphilic biosurfactants. The sugar (sophorose) head group is linked to the fatty acid hydrocarbon tail. They have been historically employed in oil spills to help mitigate their damaging environmental effect by accelerating crude oil degradation. Sophorolipids are the fermentation byproducts of the Starmarella yeasts, including Starmerella bombicola and Candida apicola. </p> |
</div> | </div> | ||
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<div class="column half_size"> | <div class="column half_size"> | ||
<h2>Product Design</h2> | <h2>Product Design</h2> | ||
| − | <p>We used the yeast Starmarella bombicola to produce sophorolipids by growing it in a yeast extract and glucose-based broth | + | <p>We used the yeast Starmarella bombicola to produce sophorolipids by growing it in a yeast extract and glucose-based broth supplemented with oleic acid. </p> |
</div> | </div> | ||
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<h2>Scale-Up and Deployment Issues | <h2>Scale-Up and Deployment Issues | ||
</h2> | </h2> | ||
| − | <p>We expect the separation and purification of the sophorolipids to incur a larger chemical cost due to the amounts of ethyl acetate and n-hexane involved in extracting water and other impurities from the solution | + | <p>We expect the separation and purification of the sophorolipids to incur a larger chemical cost due to the amounts of ethyl acetate and n-hexane involved in extracting water and other impurities from the solution in addition to separating the ethyl acetate from the sophorolipids. We also expect relatively lengthy times extracting the sophorolipids using the rotovap. |
</p> | </p> | ||
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<div class="column half_size"> | <div class="column half_size"> | ||
<h2>Environmental Impact</h2> | <h2>Environmental Impact</h2> | ||
| − | <p>Sophorolipids are | + | <p>Sophorolipids are an example of biosurfactants. These biologically based amphiphilic compounds are commonly used in the agricultural, pharmaceutical, and oil industries. The environmental benefits of biosurfactants include their low toxicity, biodegradability, and ability to enhance biodegradation and solubilization of low solubility compounds. Biosurfactants can increase the solubility and microbial biodegradability of hydrocarbons found in petroleum, reducing their devastating effects in the environment when spilled. This use of biosurfactants as dispersants applies to both aquatic environments and soil, where oil can hinder the growth of plants.</p> |
</div> | </div> | ||
<div class="column half_size"> | <div class="column half_size"> | ||
<h2>Ethics</h2> | <h2>Ethics</h2> | ||
| − | <p> | + | <p>Our project does not include testing on animals/human subjects to evaluate potential therapeutic properties of sophorolipids. Our work utilizes Starmarella bombicola (yeast) as the biological chassis to be employed as well as the organic byproducts of its fermentation. The yeast’s byproducts possess low toxicity. We also conducted the standard InterLab study using DH5-alpha E. coli. </p> |
</div> | </div> | ||
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<div class="column half_size"> | <div class="column half_size"> | ||
<h2>Safety </h2> | <h2>Safety </h2> | ||
| − | <p> | + | <p>Starmarella bombicola is also known as Candida bombicola, though Starmarella bombicola is now the preferred name. This organism is a fungus that is more specifically known as an example of a yeast. The strain has been managed in a biosafety level 2 laboratory with proper sterilization and containment procedures employed. Such devices include autoclaves, biosafety cabinets, personal protective equipment, and limited lab access. Lab safety also includes proper handling of the yeast when undergoing enzymatic modifications. </p> |
</div> | </div> | ||
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<div class="column half_size"> | <div class="column half_size"> | ||
<h2>Law and Regulation</h2> | <h2>Law and Regulation</h2> | ||
| − | <p>There are no current laws and regulations regarding the specific production or deployment of sophorolipids in the country of development. | + | <p>There are no current laws and regulations regarding the specific production or deployment of sophorolipids in the country of development. Liability laws, contingency plans, relief funds, and oil spill control mechanisms guide the responses to oil spills.</p> |
</div> | </div> | ||
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</div> | </div> | ||
<div class="column half_size"> | <div class="column half_size"> | ||
| − | < | + | <h2>Risk Assessment</h2> |
<p>Our project involves the use of sophorolipids that are biodegradable. The yeast Starmarella bombicola is not recognized as an agent associated with the transmission of a disease. </p> | <p>Our project involves the use of sophorolipids that are biodegradable. The yeast Starmarella bombicola is not recognized as an agent associated with the transmission of a disease. </p> | ||
Revision as of 19:20, 31 October 2017
| Home | Team | Collaborations | Project | Results |
| Interlab Study | Safety | Human Practices | Attributions |
Human Practices
Philosophy
Oil spills pose a sizeable threat to marine life and are classified as a major environmental disaster. Cleaning up after oil spills involves the use of dispersants. Chemical dispersants break down crude oil for easier biodegradation. This process depends on location and wind speed in addition to the use of mechanical human-guided skimmers that manually contain and collect oil. Current containment methods include surrounding the spill using floating booms to prevent further spread.
Sophorolipids (an example of biosurfactants) are natural dispersers that promote degradation of crude oil. The majority of sophorolipids are biodegradable and pose little toxicity to humans. Our team aims to create sophorolipids using the fermentation of the yeast Starmarella bombicola. We aim to improve sophorolipid production by disabling an enzyme regulating the sopholipid concentration.
Public Engagement/Dialogue
Through open communication with the community at large through social media as well as direct interaction, we aim to share knowledge regarding biosurfactants to inform the public. A two-way dialogue between our research group and the community can support the exchange of information for mutual benefits.
Education
Sophorolipids are carbohydrate-based, ampiphilic biosurfactants. The sugar (sophorose) head group is linked to the fatty acid hydrocarbon tail. They have been historically employed in oil spills to help mitigate their damaging environmental effect by accelerating crude oil degradation. Sophorolipids are the fermentation byproducts of the Starmarella yeasts, including Starmerella bombicola and Candida apicola.
Product Design
We used the yeast Starmarella bombicola to produce sophorolipids by growing it in a yeast extract and glucose-based broth supplemented with oleic acid.
Scale-Up and Deployment Issues
We expect the separation and purification of the sophorolipids to incur a larger chemical cost due to the amounts of ethyl acetate and n-hexane involved in extracting water and other impurities from the solution in addition to separating the ethyl acetate from the sophorolipids. We also expect relatively lengthy times extracting the sophorolipids using the rotovap.
Environmental Impact
Sophorolipids are an example of biosurfactants. These biologically based amphiphilic compounds are commonly used in the agricultural, pharmaceutical, and oil industries. The environmental benefits of biosurfactants include their low toxicity, biodegradability, and ability to enhance biodegradation and solubilization of low solubility compounds. Biosurfactants can increase the solubility and microbial biodegradability of hydrocarbons found in petroleum, reducing their devastating effects in the environment when spilled. This use of biosurfactants as dispersants applies to both aquatic environments and soil, where oil can hinder the growth of plants.
Ethics
Our project does not include testing on animals/human subjects to evaluate potential therapeutic properties of sophorolipids. Our work utilizes Starmarella bombicola (yeast) as the biological chassis to be employed as well as the organic byproducts of its fermentation. The yeast’s byproducts possess low toxicity. We also conducted the standard InterLab study using DH5-alpha E. coli.
Safety
Starmarella bombicola is also known as Candida bombicola, though Starmarella bombicola is now the preferred name. This organism is a fungus that is more specifically known as an example of a yeast. The strain has been managed in a biosafety level 2 laboratory with proper sterilization and containment procedures employed. Such devices include autoclaves, biosafety cabinets, personal protective equipment, and limited lab access. Lab safety also includes proper handling of the yeast when undergoing enzymatic modifications.
Law and Regulation
There are no current laws and regulations regarding the specific production or deployment of sophorolipids in the country of development. Liability laws, contingency plans, relief funds, and oil spill control mechanisms guide the responses to oil spills.
Risk Assessment
Our project involves the use of sophorolipids that are biodegradable. The yeast Starmarella bombicola is not recognized as an agent associated with the transmission of a disease.
