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<p style="margin-top: 10px;">At the beginning, we read a flood of literature, and we found that rare earth elements, especially the capture methods, attracted numerous scholars both at home and abroad to explore. They have already constructed successful genetically encoded sensors for some rare earth elements. However, the lanthanide elements,one of the important and wealthy rare earth elements,are not included. Consequently, this year,our team are eager to fill the research vacancy.</p> | <p style="margin-top: 10px;">At the beginning, we read a flood of literature, and we found that rare earth elements, especially the capture methods, attracted numerous scholars both at home and abroad to explore. They have already constructed successful genetically encoded sensors for some rare earth elements. However, the lanthanide elements,one of the important and wealthy rare earth elements,are not included. Consequently, this year,our team are eager to fill the research vacancy.</p> | ||
<p>We want to construct an intelligent system. Only when the concentration of the lanthanide ions reach the threshold, does it react. </p> | <p>We want to construct an intelligent system. Only when the concentration of the lanthanide ions reach the threshold, does it react. </p> | ||
− | <p>In order to reach our goal, we need a smart sensing circuit. Fortunately,we found a two-component system called | + | <p>In order to reach our goal, we need a smart sensing circuit. Fortunately,we found a two-component system called PmrA/PmrB. This system itself is aimed at detecting iron ions. If we can find appropriate sequence which can react to lanthanide ions, we can replace the iron-binding sequence with them to achieve our goal. |
− | <p> Through consulting a large number of literature, we made it! We know the most successful lanthanide binder is lanthanide-binding tag.And we will call it LBT in the following. After exchanging sequences, we fuse the pmrC promoter to the <I>gfp</I> gene which encodes green fluorescent protein to prove whether or not the | + | <p> Through consulting a large number of literature, we made it! We know the most successful lanthanide binder is lanthanide-binding tag.And we will call it LBT in the following. After exchanging sequences, we fuse the pmrC promoter to the <I>gfp</I> gene which encodes green fluorescent protein to prove whether or not the PmrA-PmrB(LBT) are responsive to lanthanide.</p> |
<p>Ions sensing is just the first step, then we need to capture them. In this part, we also use LBT——our good lanthanide hunter.</p> | <p>Ions sensing is just the first step, then we need to capture them. In this part, we also use LBT——our good lanthanide hunter.</p> | ||
<p>In order to capture more efficiently, we repeat them three times on cell with GS-Linker between each.</p> | <p>In order to capture more efficiently, we repeat them three times on cell with GS-Linker between each.</p> |
Revision as of 19:27, 31 October 2017