Difference between revisions of "Team:Arizona State/Improve"

For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.

Gold Medal Criterion #2

Standard Tracks: Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.

Special Tracks: Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.

Summary for Lux Receiver:

When testing the LuxR the AubI showed a higher expression when mixed with 10% of a second sender (even when that sender was a negative control sender). The 40% AubI mixed with 10% negative sender and the 40% AubI mixed with 10% RhlI both expressed higher than the 50% AubI by itself. This result was confirmed in another test where 40% Aub mixed with 10% EsaI and 40% AubI mixed with 10% CerI both expressed higher than the 50% AubI alone. (See graphs below).

Revision as of 20:04, 31 October 2017 (view source) Clsmit52 (Talk \| contribs) ← Older edit		Revision as of 20:04, 31 October 2017 (view source) Clsmit52 (Talk \| contribs) Newer edit →
Line 16:		Line 16:
	<p>When testing the LuxR the AubI showed a higher expression when mixed with 10% of a second sender (even when that sender was a negative control sender). The 40% AubI mixed with 10% negative sender and the 40% AubI mixed with 10% RhlI both expressed higher than the 50% AubI by itself. This result was confirmed in another test where 40% Aub mixed with 10% EsaI and 40% AubI mixed with 10% CerI both expressed higher than the 50% AubI alone. (See graphs below).</p>		<p>When testing the LuxR the AubI showed a higher expression when mixed with 10% of a second sender (even when that sender was a negative control sender). The 40% AubI mixed with 10% negative sender and the 40% AubI mixed with 10% RhlI both expressed higher than the 50% AubI by itself. This result was confirmed in another test where 40% Aub mixed with 10% EsaI and 40% AubI mixed with 10% CerI both expressed higher than the 50% AubI alone. (See graphs below).</p>

−	<center><img src="https://2017.igem.org/File:A4.png ~~" alt="Design Flowchart~~" style="max-width: 600px; width: 80%"></center>	+	<center><img src="https://2017.igem.org/File:A4.png " style="max-width: 600px; width: 80%"></center>