Difference between revisions of "Team:MSU-Michigan/Measurement"
| Line 73: | Line 73: | ||
$('.myDIV0').hover( //when you hover over the figure | $('.myDIV0').hover( //when you hover over the figure | ||
function() { | function() { | ||
| − | $(this).append($("<img id='char0' src='https://static.igem.org/mediawiki/2017/thumb/c/c8/MSU-Michigan_gfp_maturation_description.png/800px-MSU-Michigan_gfp_maturation_description.png' />").hide().fadeIn()); //show the character | + | $(this).append($("<img id='char0' src='https://static.igem.org/mediawiki/2017/thumb/c/c8/MSU-Michigan_gfp_maturation_description.png/800px-MSU-Michigan_gfp_maturation_description.png'/>").hide().fadeIn()); //show the character |
}, function() { | }, function() { | ||
$('#char0').remove(); //get rid of it after moving the cursor away from the figure | $('#char0').remove(); //get rid of it after moving the cursor away from the figure | ||
| Line 80: | Line 80: | ||
</script> | </script> | ||
| − | < | + | <div class="myDIV0" style="position:left"> |
| − | + | <img class="two" src='img src="https://static.igem.org/mediawiki/2017/5/5e/MSU-Michigan_current_gfp%2Cgfpmtrb.png'/> | |
| − | + | </div> | |
| − | + | ||
| − | + | ||
| − | <img src="https://static.igem.org/mediawiki/2017/5/5e/MSU-Michigan_current_gfp%2Cgfpmtrb.png | + | |
| − | </ | + | |
<style> | <style> | ||
html, body { | html, body { | ||
Revision as of 22:48, 31 October 2017
Measurement
Using Current for Protein Expression
MSU-iGEM 2017 developed a measurement tool that researchers throughout synthetic biology can utilize. Green fluorescent proteins (GFP) are used under aerobic conditions to signal protein expression. Unfortunately, for researchers working under anaerobic conditions, GFP does not mature and thus fluoresce under anaerobic conditions. Therefore, we showed that current induced via IPTG produces a reproducible signal for protein expression under anaerobic conditions. Current induction occurs 18 min after IPTG is added displaying a more rapid response than GFP expression. This measurement tool even proves to be more efficient than GFP expression under aerobic conditions due to the rapid current increased after induction. Also, current can be measured using simpler measuring tools than GFP expression. MSU-iGEM’s simplistic bioreactors utilizing Arduino boards are a model example of a system that can quantitatively measure protein expression via current increase in a more versatile way than using tools for measuring GFP fluorescence.
/*character for gfp maturation*/![](img src="https://static.igem.org/mediawiki/2017/5/5e/MSU-Michigan_current_gfp%2Cgfpmtrb.png)
