Difference between revisions of "Team:Virginia/Improve"
(Updating Improved BioBrick Wiki Description.) |
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<li><a href="http://parts.igem.org/Part:BBa_K1067002">BBa_K1067002</a></li> | <li><a href="http://parts.igem.org/Part:BBa_K1067002">BBa_K1067002</a></li> | ||
<li><a href="http://parts.igem.org/Part:BBa_K1067004">BBa_K1067004</a></li> | <li><a href="http://parts.igem.org/Part:BBa_K1067004">BBa_K1067004</a></li> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/d/d7/Virginia_dtu_cyt.png"> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/d/d1/Virginia_dtu_amo.png"> | ||
</ul> | </ul> | ||
<br></br> | <br></br> | ||
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<p>Improved part from Virginia 2017: | <p>Improved part from Virginia 2017: | ||
<a href="http://parts.igem.org/Part:BBa_K2272019">BBa_K2272019</a></p> | <a href="http://parts.igem.org/Part:BBa_K2272019">BBa_K2272019</a></p> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/4/44/Virginia_pvhb_hao_cyt.png"> | ||
Revision as of 07:23, 1 November 2017
Improve
The two parts from iGEM13_DTU-Denmark listed above are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting condionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.
Improved part from Virginia 2017: BBa_K2272019
