Difference between revisions of "Team:NTHU Taiwan/Composite Part"
Steven870202 (Talk | contribs) |
|||
| Line 20: | Line 20: | ||
display:block; | display:block; | ||
float:left; | float:left; | ||
| − | font-family:' | + | font-family:'Helvetica', sans-serif; |
background-color: #F6F6E3; | background-color: #F6F6E3; | ||
} | } | ||
| Line 39: | Line 39: | ||
.igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 { | .igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 { | ||
| − | font-family: | + | font-family: Helvetica; |
padding: 50px 0px 15px 0px; | padding: 50px 0px 15px 0px; | ||
border-bottom: 0px; | border-bottom: 0px; | ||
| Line 54: | Line 54: | ||
.igem_2017_content_wrapper .content p{ | .igem_2017_content_wrapper .content p{ | ||
| − | font-family: | + | font-family: Helvetica; |
color: #2C2C2C; | color: #2C2C2C; | ||
font-size: 1.4em; | font-size: 1.4em; | ||
Latest revision as of 16:09, 1 November 2017
Composite Parts
We constructed three composite parts and all of their function have been validated:Results
| Biobrick | Type | Name | Description | Designer | Length |
| K2354010 | Composite | monobody-GBP HS | Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface. | Chan, Wei-Jen | 797 |
| K2354011 | Composite | INP-ER-alpha HS | Expression of ER-alpha on the membrane of E. coli and ER-alpha can bind to EDCs | Chan, Wei-Jen | 2199 |
| K2354012 | Composite | HRP-his tag | Degrade Estrogen Disrupt Chemicals | Chan, Wei-Jen | 1217 |
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and monobody can bind to ER-alpha/EDCs complex.
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of E. coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.
Horseradish peroxidase is a kind of peroxidase which uses heme as it's cofactor, and it can degrade EDCs to less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
