Difference between revisions of "Team:Virginia/Improve"

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<h1>Improve</h1>
 
<h1>Improve</h1>
 
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<li><a href="http://parts.igem.org/Part:BBa_K1067002">BBa_K1067002</a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K1067004">BBa_K1067004</a></li>
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The two parts from iGEM13 DTU-Denmark, <a href="http://parts.igem.org/Part:BBa_K1067002" width="100" height="100">BBa_K1067002</a> and <a href="http://parts.igem.org/Part:BBa_K1067004" width="100" height="100">BBa_K1067004</a>, are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B.  HAO-B is a putative protein, that at the time may not have been believed to be significant.  However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting conditionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.</p>
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<img src="https://static.igem.org/mediawiki/parts/d/d7/Virginia_dtu_cyt.png">
 
<img src="https://static.igem.org/mediawiki/parts/d/d7/Virginia_dtu_cyt.png">
 
<img src="https://static.igem.org/mediawiki/parts/d/d1/Virginia_dtu_amo.png">
 
<img src="https://static.igem.org/mediawiki/parts/d/d1/Virginia_dtu_amo.png">
 
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The two parts from iGEM13_DTU-Denmark listed above are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B.  HAO-B is a putative protein, that at the time may not have been believed to be significant.  However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting condionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.</p>
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<p>Improved part from Virginia 2017:
 
<p>Improved part from Virginia 2017:

Revision as of 14:46, 1 November 2017


Improve



    The two parts from iGEM13 DTU-Denmark, BBa_K1067002 and BBa_K1067004, are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting conditionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.





Improved part from Virginia 2017: BBa_K2272019