Difference between revisions of "Team:Hong Kong UCCKE/Results"
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| − | <p style="text-align:left !important;"> | + | <p style="text-align:left !important;">After we have successfully cloned our plasmids into the E.coli, we did miniprep to obtain purified plasmid DNA. Then, we did restriction map and have sent the samples to BGI for sequencing. The results are shown below.</p> |
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<p><font size="4">Sequencing Result</font></p> | <p><font size="4">Sequencing Result</font></p> | ||
| − | <p style="text-align:centre !important;"> With the help of | + | <p style="text-align:centre !important;"> With the help of CUHK, we sent our plasmid to BGI for sequencing. We aligned the sequence that we ordered from IDT(As the template sequence 99593011 below) with the sequencing result received from BGI(as the aligned sequence below). And here is our analysis.</p> |
<p style="text-align:centre !important;"> The black area indicates that the two sequence are the same which those red strip represent that there're some mutations.</p> | <p style="text-align:centre !important;"> The black area indicates that the two sequence are the same which those red strip represent that there're some mutations.</p> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2017/c/c6/T--Hong_Kong_UCCKE--300sequence.jpg" alt="300 sequencing resultl"style="width:65%;"> | <img src="https://static.igem.org/mediawiki/2017/c/c6/T--Hong_Kong_UCCKE--300sequence.jpg" alt="300 sequencing resultl"style="width:65%;"> | ||
</a> | </a> | ||
| − | <br><span class="imgcaption"> | + | <br><span class="imgcaption">Sequencing result of BBa_K2197300</span> |
</div><br> | </div><br> | ||
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| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
<div class="row pop"> | <div class="row pop"> | ||
<div class="col-xs-6"> | <div class="col-xs-6"> | ||
| − | <a href="https://static.igem.org/mediawiki/2017/1/1b/T--Hong_Kong_UCCKE--_project_300_screenshot_1.png" title=" | + | <a href="https://static.igem.org/mediawiki/2017/1/1b/T--Hong_Kong_UCCKE--_project_300_screenshot_1.png" title="Allignment (1)"><img src="https://static.igem.org/mediawiki/2017/1/1b/T--Hong_Kong_UCCKE--_project_300_screenshot_1.png" style="width:100%;"/></a> |
| − | <br><span class="imgcaption"> | + | <br><span class="imgcaption">Sequence Alignment(1)</span> |
</div> | </div> | ||
<div class="col-xs-6"> | <div class="col-xs-6"> | ||
<a href="https://static.igem.org/mediawiki/2017/1/16/T--Hong_Kong_UCCKE--_project_300_screenshot_2.png" title="Project 300 (2)"><img src="https://static.igem.org/mediawiki/2017/1/16/T--Hong_Kong_UCCKE--_project_300_screenshot_2.png" style="width:100%;"/></a> | <a href="https://static.igem.org/mediawiki/2017/1/16/T--Hong_Kong_UCCKE--_project_300_screenshot_2.png" title="Project 300 (2)"><img src="https://static.igem.org/mediawiki/2017/1/16/T--Hong_Kong_UCCKE--_project_300_screenshot_2.png" style="width:100%;"/></a> | ||
| − | <br><span class="imgcaption"> | + | <br><span class="imgcaption">Sequence Alignment(2)</span> |
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <div class="row"><div class="col-md-20"> | ||
| + | <p><font size="4">Conclusion</font></p> | ||
| + | <p style="text-align:left !important;"> From the results above, we can say we successfully clone the DNA that IDT sent us into the cell. However, as what we have mentioned in the experiment page, we found that the HucO operate is missing. Then, we aligned the sequence registered in IGEM with the one that IDT send us. </p> | ||
| + | </div> | ||
| + | <div class="row pop"> | ||
| + | <div class="col-xs-6"> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/c/ca/T--Hong_Kong_UCCKE--sequenceofigem2.jpg" title="Alignment (1)"><img src="https://static.igem.org/mediawiki/2017/c/ca/T--Hong_Kong_UCCKE--sequenceofigem2.jpg" style="width:100%;"/></a> | ||
| + | <br><span class="imgcaption">Sequence Alignment(1)</span> | ||
| + | </div> | ||
| + | <div class="col-xs-6"> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/7/75/T--Hong_Kong_UCCKE--sequenceofigem1.jpg" title="Alignment (2)"><img src="https://static.igem.org/mediawiki/2017/7/75/T--Hong_Kong_UCCKE--sequenceofigem1.jpg" style="width:100%;"/></a> | ||
| + | <br><span class="imgcaption">Sequence Alignment(2)</span> | ||
| + | </div> | ||
| + | </div><br> | ||
<div class="row"><div class="col-md-20"> | <div class="row"><div class="col-md-20"> | ||
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<div class="pop"> | <div class="pop"> | ||
<a href="https://static.igem.org/mediawiki/2017/c/c1/T--Hong_Kong_UCCKE--gelsequence400.png" style="width:65%;"/><img src="https://static.igem.org/mediawiki/2017/c/c1/T--Hong_Kong_UCCKE--gelsequence400.png" style="width:65%;"/></a> | <a href="https://static.igem.org/mediawiki/2017/c/c1/T--Hong_Kong_UCCKE--gelsequence400.png" style="width:65%;"/><img src="https://static.igem.org/mediawiki/2017/c/c1/T--Hong_Kong_UCCKE--gelsequence400.png" style="width:65%;"/></a> | ||
| − | <br><span class="imgcaption"> | + | <br><span class="imgcaption">Sequencing Result of BBa_K2197400</span> |
<br> | <br> | ||
</div> | </div> | ||
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<div class="col-xs-6"> | <div class="col-xs-6"> | ||
<a href="https://static.igem.org/mediawiki/2017/2/2d/T--Hong_Kong_UCCKE--_project_400_screenshot_1.png" title="Project 400 (1)"><img src="https://static.igem.org/mediawiki/2017/2/2d/T--Hong_Kong_UCCKE--_project_400_screenshot_1.png" style="width:100%;"/></a> | <a href="https://static.igem.org/mediawiki/2017/2/2d/T--Hong_Kong_UCCKE--_project_400_screenshot_1.png" title="Project 400 (1)"><img src="https://static.igem.org/mediawiki/2017/2/2d/T--Hong_Kong_UCCKE--_project_400_screenshot_1.png" style="width:100%;"/></a> | ||
| − | <br><span class="imgcaption"> | + | <br><span class="imgcaption">Sequence Alignment (1)</span> |
</div> | </div> | ||
<div class="col-xs-6"> | <div class="col-xs-6"> | ||
<a href="https://static.igem.org/mediawiki/2017/e/e1/T--Hong_Kong_UCCKE--_project_400_screenshot_2.png" title="Project 400 (2)"><img src="https://static.igem.org/mediawiki/2017/e/e1/T--Hong_Kong_UCCKE--_project_400_screenshot_2.png" style="width:100%;"/></a> | <a href="https://static.igem.org/mediawiki/2017/e/e1/T--Hong_Kong_UCCKE--_project_400_screenshot_2.png" title="Project 400 (2)"><img src="https://static.igem.org/mediawiki/2017/e/e1/T--Hong_Kong_UCCKE--_project_400_screenshot_2.png" style="width:100%;"/></a> | ||
| − | <br><span class="imgcaption"> | + | <br><span class="imgcaption">Sequence Alignment (2)</span> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 12:13, 1 November 2017
After we have successfully cloned our plasmids into the E.coli, we did miniprep to obtain purified plasmid DNA. Then, we did restriction map and have sent the samples to BGI for sequencing. The results are shown below.
BBa_K2197300
Restriction Map
After mini prep, we use the same restriction enzyme EcoR1 and Pst1 to do restriction digestion and gel electrophoresis. However, the size of K2197300 and the backbone pSB1C3 is almost the same, thus we cannot prove by the Gel photo.
Image of gel electrophoresis of 300a+b.
Sequencing Result
With the help of CUHK, we sent our plasmid to BGI for sequencing. We aligned the sequence that we ordered from IDT(As the template sequence 99593011 below) with the sequencing result received from BGI(as the aligned sequence below). And here is our analysis.
The black area indicates that the two sequence are the same which those red strip represent that there're some mutations.
Sequencing result of BBa_K2197300
Sequence Alignment(1)
Sequence Alignment(2)
Conclusion
From the results above, we can say we successfully clone the DNA that IDT sent us into the cell. However, as what we have mentioned in the experiment page, we found that the HucO operate is missing. Then, we aligned the sequence registered in IGEM with the one that IDT send us.
Sequence Alignment(1)
Sequence Alignment(2)
Project 400
Restriction Map
After mini prep, we use the same restriction enzyme EcoR1 and Pst1 to do restriction digestion and gel electrophoresis. As seen, the size of K2197400 (2214 bp) and the backbone pSB1C3 (2070 bp)is almost the same, similar to our predicted result.
Image of gel electrophoresis of 400a+b and 500
Sequencing Result
With the help of HKCU, we sent our plasmid to BGI for sequencing. We aligned the sequence that we ordered from IDT(As the template sequence 99593011 below) with the sequencing result received from BGI(as the aligned sequence below). And here is our analysis.
The black area indicates that the two sequence are the same which those red strip represent that there're some mutations.
Sequence Alignment (1)
Sequence Alignment (2)
Project 500
Restriction Map
Also using the same restriction enzyme EcoR1 and Pst1 to do restriction digestion and gel electrophoresis. The size of K2197500 (1668 bp) and the backbone pSB1C3 (2070 bp).
Image of gel electrophoresis of 400a+b and 500
Image of theoretical gel electrophoresis of 500 from genome compiler
Sequencing Result
Sequencing Result
With the help of HKCU, we sent our plasmid to BGI for sequencing. We aligned the sequence that we ordered from IDT(As the template sequence 99593011 below) with the sequencing result received from BGI(as the aligned sequence below). And here is our analysis.
The black area indicates that the two sequence are the same which those red strip represent that there're some mutations.
Project 500
Project 500 (1)
Project 500 (2)
Project 502
Project 502 (1)
Project 502 (2)
