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Second, we amplify gene A by primer 1 & 2 and amplify gene B by primer 3 & 4 respectively. After that we can get extended gene A and gene B. | Second, we amplify gene A by primer 1 & 2 and amplify gene B by primer 3 & 4 respectively. After that we can get extended gene A and gene B. | ||
Last but not least, we blend extended gene A & gene B can complemet each other and form the hybirdized strands. Under the function of DNA polymerase I, gene A and gene B can be the primer and templet of each other. After PCR, we can get fused Gene A-B.</p> | Last but not least, we blend extended gene A & gene B can complemet each other and form the hybirdized strands. Under the function of DNA polymerase I, gene A and gene B can be the primer and templet of each other. After PCR, we can get fused Gene A-B.</p> | ||
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Revision as of 13:09, 1 November 2017