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carried out engineering modification of this enzyme to improve the catalytic effect of the core part. | carried out engineering modification of this enzyme to improve the catalytic effect of the core part. | ||
We used the AEMD (Auto Enzyme Mutation Design) platform to identify mutational sites and screened | We used the AEMD (Auto Enzyme Mutation Design) platform to identify mutational sites and screened | ||
− | for high catalytic efficiency by HPLC (High Performance Liquid Chromatography | + | for high catalytic efficiency by HPLC (High Performance Liquid Chromatography) and HTS (High throughput screening) of the ceaS2 mutant. |
− | + | ||
<br><br> In the selection of the chassis organisms, we chose E. coli which is a kind of classic chassis organism | <br><br> In the selection of the chassis organisms, we chose E. coli which is a kind of classic chassis organism | ||
in prokaryote and Saccharomyces cerevisiae which is the most easily manipulated chassis organism | in prokaryote and Saccharomyces cerevisiae which is the most easily manipulated chassis organism |
Revision as of 14:41, 1 November 2017