Difference between revisions of "Team:Berlin diagnostX"
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<p class="text-justify mb-4">We identified 215 unique, potential diagnost-x targets.</p> | <p class="text-justify mb-4">We identified 215 unique, potential diagnost-x targets.</p> | ||
<p class="text-justify mb-4">The colour intensity is proportional to the expression of each identified possible target.</p> | <p class="text-justify mb-4">The colour intensity is proportional to the expression of each identified possible target.</p> | ||
| − | <img class="d-inline pfeilg" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | + | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> |
<p class="d-inline pl-3"> First direct RNA isolation (blau) from T. solium eggs without a manipulative hatching procedure and thereof performed transcriptome analysis </p> | <p class="d-inline pl-3"> First direct RNA isolation (blau) from T. solium eggs without a manipulative hatching procedure and thereof performed transcriptome analysis </p> | ||
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | ||
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<h5 class="igem_blue pb-1">Sensor Design</h5> | <h5 class="igem_blue pb-1">Sensor Design</h5> | ||
<p class="text-justify mb-4">Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.</p> | <p class="text-justify mb-4">Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.</p> | ||
| − | <img class="d-inline pfeilg" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | + | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> |
<p class="d-inline pl-3"> Refined preselection of promising sensors thanks to established software</p> | <p class="d-inline pl-3"> Refined preselection of promising sensors thanks to established software</p> | ||
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | ||
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<h5 class="igem_blue pb-1">Sensor Screening</h5> | <h5 class="igem_blue pb-1">Sensor Screening</h5> | ||
<p class="text-justify mb-4">The designed switches from the In Silico Team were furthermore assembled using Extension PCR with for every switch tailored primers. Assembled Sensors passed all through the standard screening in liquid phase.</p> | <p class="text-justify mb-4">The designed switches from the In Silico Team were furthermore assembled using Extension PCR with for every switch tailored primers. Assembled Sensors passed all through the standard screening in liquid phase.</p> | ||
| − | <img class="d-inline pfeilg" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | + | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> |
<p class="d-inline pl-3"> 6 successfully working sensors detected thanks to our high flow-through Screening Pipeline</p> | <p class="d-inline pl-3"> 6 successfully working sensors detected thanks to our high flow-through Screening Pipeline</p> | ||
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | ||
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<h5 class="igem_blue pb-1">Considerating future application from Day 1</h5> | <h5 class="igem_blue pb-1">Considerating future application from Day 1</h5> | ||
<p class="text-justify mb-4">Neglected Tropical Diseases...</p> | <p class="text-justify mb-4">Neglected Tropical Diseases...</p> | ||
| − | <img class="d-inline pfeilg" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | + | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> |
<p class="d-inline pl-3"> Punkt 1 </p> | <p class="d-inline pl-3"> Punkt 1 </p> | ||
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | ||
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<h5 class="igem_blue pb-1">Raising awareness for T. solium </h5> | <h5 class="igem_blue pb-1">Raising awareness for T. solium </h5> | ||
<p class="text-justify mb-4">Neglected Tropical Diseases have few platforms in the eastern world since they don't affect people directly. We saw our mission also to a big extend in the fundamental raising of awareness for NTDs and education of a new generation.</p> | <p class="text-justify mb-4">Neglected Tropical Diseases have few platforms in the eastern world since they don't affect people directly. We saw our mission also to a big extend in the fundamental raising of awareness for NTDs and education of a new generation.</p> | ||
| − | <img class="d-inline pfeilg" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | + | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> |
<p class="d-inline pl-3">Organisation of a congress with <strong>steakholders & 150 participants</strong> amongst many other activities</p> | <p class="d-inline pl-3">Organisation of a congress with <strong>steakholders & 150 participants</strong> amongst many other activities</p> | ||
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | ||
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<p class="text-justify mb-4">The 21st century leaves it's marks - new methods for intelligent assisted education are wanted - and we're on the track of time! | <p class="text-justify mb-4">The 21st century leaves it's marks - new methods for intelligent assisted education are wanted - and we're on the track of time! | ||
Dynamic & new ways of solving problems will determine the success of social entrepreneurship - get started!</p> | Dynamic & new ways of solving problems will determine the success of social entrepreneurship - get started!</p> | ||
| − | <img class="d-inline pfeilg" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | + | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> |
<p class="d-inline pl-3">Development of an <strong>augmented reality software tool</strong> for innovative future education</p> | <p class="d-inline pl-3">Development of an <strong>augmented reality software tool</strong> for innovative future education</p> | ||
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="#" role="button">Discover More</a></div> | ||
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Revision as of 14:37, 1 November 2017
The fight against T. solium
The worldwide fight against T.solium is facing three different challenges
HIGH PREVALENCE
Estimated billions are affected, the infection causing epilepsy, blindness and death
INSUFFICIENT DRUG DISTRIBUTION
Although affordable therapeutics exit
EXPENSIVE DIAGNOSTICS
No method suited for field use
The vision of diagnost-x
Developing a low cost, easy-to-handle diagnostic test for infection with the tapeworm Taenia solium
Target Identification
Having 96 million RNAs sequenced and mapped them against about 12.000 Genes of T. saginata T. asiatica and T. solium, the expression level of unique RNAs was analyzed.
We identified 215 unique, potential diagnost-x targets.
The colour intensity is proportional to the expression of each identified possible target.
First direct RNA isolation (blau) from T. solium eggs without a manipulative hatching procedure and thereof performed transcriptome analysis
Sensor Design
Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.
Refined preselection of promising sensors thanks to established software
Sensor Screening
The designed switches from the In Silico Team were furthermore assembled using Extension PCR with for every switch tailored primers. Assembled Sensors passed all through the standard screening in liquid phase.
6 successfully working sensors detected thanks to our high flow-through Screening Pipeline
Raising awareness for T. solium
Neglected Tropical Diseases have few platforms in the eastern world since they don't affect people directly. We saw our mission also to a big extend in the fundamental raising of awareness for NTDs and education of a new generation.
Organisation of a congress with steakholders & 150 participants amongst many other activities
Route to the Future
The 21st century leaves it's marks - new methods for intelligent assisted education are wanted - and we're on the track of time! Dynamic & new ways of solving problems will determine the success of social entrepreneurship - get started!
Development of an augmented reality software tool for innovative future education
