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<li><a href="https://static.igem.org/mediawiki/2017/8/85/T--Bielefeld-CeBiTec--protocol_50xTAE.pdf" target="blank">50xTAE Stock Solution</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/8/85/T--Bielefeld-CeBiTec--protocol_50xTAE.pdf" target="blank">50xTAE Stock Solution</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/1/15/T--Bielefeld-CeBiTec--protocol_acetone.pdf" target="blank">Acetone Precipitation</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/1/15/T--Bielefeld-CeBiTec--protocol_acetone.pdf" target="blank">Acetone Precipitation</a></li> | ||
− | <li><a href="https://static.igem.org/mediawiki/2017/ | + | <li><a href="https://static.igem.org/mediawiki/2017/8/8c/T--Bielefeld-CeBiTec--YKE_lycopene_protocol.pdf" target="blank">Lycopene extraction</a></li> |
<li><a href="https://static.igem.org/mediawiki/2017/3/3a/T--Bielefeld-CeBiTec--protocol_blunt-end-ligation.pdf" target="blank">Blunt end ligation</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/3/3a/T--Bielefeld-CeBiTec--protocol_blunt-end-ligation.pdf" target="blank">Blunt end ligation</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/c/cc/T--Bielefeld-CeBiTec--protocol_colonyPCR_GoTaq_G2.pdf" target="blank">Colony PCR GoTaq® G2 DNA Polymerase</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/c/cc/T--Bielefeld-CeBiTec--protocol_colonyPCR_GoTaq_G2.pdf" target="blank">Colony PCR GoTaq® G2 DNA Polymerase</a></li> |
Revision as of 15:17, 1 November 2017
![](https://static.igem.org/mediawiki/2017/7/74/T--Bielefeld-CeBiTec--title-img-centrifuge.jpg)
Methods
Protocols
- 50xTAE Stock Solution
- Acetone Precipitation
- Lycopene extraction
- Blunt end ligation
- Colony PCR GoTaq® G2 DNA Polymerase
- Transformation via electroporation
- Fast cell lysis sample buffer
- Fast cell lysis for SDS-PAGE
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- Generating chemocompetent cells
- Generating electrocompetent cells
- Gibson Assembly
- Transformation via heat shock
- innuPREP Gel Extraktion Kit (Analytik Jena)
- Lysogeny Broth (LB)
- Cloning Strategy with Restriction Enzymes
- Biotinylation of glass slides
- Plasmid Assembly Protocol with Golden Gate Assembly
- Monarch® Plasmid Miniprep Kit (NEB)
- Monarch® PCR & DNA Cleanup Kit (NEB)
- MOPS Minimal Medium
- Native DNA PAGE
- PBJR buffer
- PCR with Q5 High-Fidelity Polymerase
- PCR with Phusion High Fidelity Master Mix with HC enhancer
- Wizard® SV Gel and PCR Clean-Up System (Promega)
- PureYieldTM Plasmid Miniprep System (Promega)
- "Typical" Restriction Digest
- SDS-PAGE running buffer
- SOC-medium
- ssDNA Annealing
- Standard BioBrick Assembly
- TAE buffer
- ZymoPURETM Plasmid Miniprep Kit (Zymo Research)
- SDS-PAGE
- Genome editing via the CRISP-Cas9 system
- Protein Purification Impact Protocol
- Bradford estimation using RotiNanoquant
Enzymes
Digestion enzymes
Enzyme | Producer |
---|---|
AgeI-HF | NEB |
BsaI | NEB |
DpnI | NEB |
DpnI | Jena Bioscience |
EciI | NEB |
EcoRV | Fermentas |
MnlI | NEB |
NotI | Jena Bioscience |
PstI | NEB |
PstI | Thermo Fisher Scientific |
PstI-HF | NEB |
SapI | NEB |
SpeI | NEB |
XbaI | NEB |
XbaI | Jena Bioscience |
Polymerases
Enzyme | Producer |
---|---|
Go Taq DNA polymerase | Promega |
Klenow Fragment | Thermo Fisher Scientific |
Phusion High-Fidelity DNA polymerase | NEB |
Q5 High-Fidelity DNA polymerase | NEB |
Taq Polymerase | Jena Bioscience |
Other Enzymes
Enzyme | Producer |
---|---|
Shrimp Alkaline Phosphatase | Jena Bioscience |
T4 DNA Ligase | NEB |
T4 DNA Ligase | Thermo Fisher Scientific |
T4 Polynucleotide Kinase | NEB |