Difference between revisions of "Team:UCLouvain/Composite Part"
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</div> | </div> | ||
<section> | <section> | ||
| − | + | <div class="container"> | |
<div class="row" style="margin-bottom: 40px;"> | <div class="row" style="margin-bottom: 40px;"> | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | <span> | + | <span>Parts we created</span> |
</div> | </div> | ||
<BR><BR> | <BR><BR> | ||
<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | <p class="montserrat-text uppercase">Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.</p> | + | <p class="montserrat-text uppercase">pCMMCD 105</p> |
| − | + | </div> | |
| − | + | ||
| − | </p> | + | <p>Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.</p> |
| − | + | ||
| − | </p> | + | <p>GusA encodes the <i>beta</i>-glucuronidase gene from <i>E. coli</i>. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and <i>para</i>-nitrophenyl glucuronide.</p> |
| − | < | + | |
| + | <p>Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).</p> | ||
| + | |||
| + | <BR><BR> | ||
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<div class="container"> | <div class="container"> | ||
<div class="row" style="margin-bottom: 40px;"> | <div class="row" style="margin-bottom: 40px;"> | ||
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<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | <span> | + | <span>Parts we used</span> |
</div> | </div> | ||
<BR><BR> | <BR><BR> | ||
<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | <p class="montserrat-text uppercase">iGEM plasmid consisting of araC, a pBAD/AraC promoter (I0500), a RBS(B0034), a RFP(E1010) and two terminators(B0010 and B0012) contained inside a same biobrick (B0015) | + | <p class="montserrat-text uppercase">piFinal <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_K577882">(BBa_K577882)</a></p> |
| − | + | </div> | |
| + | |||
| + | <p>iGEM plasmid consisting of araC, a pBAD/AraC promoter (I0500), a RBS(B0034), a RFP(E1010) and two terminators(B0010 and B0012) contained inside a same biobrick (B0015)</p> | ||
| + | |||
| + | |||
| + | </div> | ||
Revision as of 16:47, 1 November 2017
in the lab
Parts we created
pCMMCD 105
Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.
GusA encodes the beta-glucuronidase gene from E. coli. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and para-nitrophenyl glucuronide.
Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).
Parts we used
piFinal (BBa_K577882)
iGEM plasmid consisting of araC, a pBAD/AraC promoter (I0500), a RBS(B0034), a RFP(E1010) and two terminators(B0010 and B0012) contained inside a same biobrick (B0015)
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