Difference between revisions of "Team:UCLouvain/Basic Part"
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<span>Parts we created</span> | <span>Parts we created</span> | ||
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<p class="montserrat-text uppercase">ComR (BBa_K2341000)</p> | <p class="montserrat-text uppercase">ComR (BBa_K2341000)</p> | ||
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| − | + | <p>Protein originally found in <i>Streptococcus thermophilius</i> where it regulates natural competence. The protein activation and binding to specific promoters (ComR Box; BBa_K2341001) is only possible in presence of a short peptide, ComS (LPYFAGCL). Binding to the promoter will induce the downstream sequence expression.</p> | |
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| − | + | <p>Promote expression of the sequence downstream in presence of ComR (BBa_K2341000) and ComS (LPYFAGCL). Sequence containing the palindrome motif needed to bind ComR (= ComR box).</p> | |
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<p class="montserrat-text uppercase">sgRNA Tyrosin (Cas9 guide) (BBa_K2341100)</p> | <p class="montserrat-text uppercase">sgRNA Tyrosin (Cas9 guide) (BBa_K2341100)</p> | ||
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| − | + | <p>RNA guide to use in combination with CRISPR/CAS9. Cut inside the tyrA gene so that deletion by homologous recombination can occur if a homologous DNA strand is also added. RNA guide under constitutive expression by BBa_J23119.</p> | |
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<span>Parts we used</span> | <span>Parts we used</span> | ||
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<p class="montserrat-text uppercase">pBAD/araC (I0500)</p> | <p class="montserrat-text uppercase">pBAD/araC (I0500)</p> | ||
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| − | + | <p>The pBAD promoter is a very common inducible one that is activated by L-arabinose and repressed by araC.</p> | |
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<p class="montserrat-text uppercase">Terminator (B0010)</p> | <p class="montserrat-text uppercase">Terminator (B0010)</p> | ||
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| − | + | <p>Terminators are necessary to stop the transcription at the end of a coding sequence.</p> | |
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| − | + | <p>Efficient ribosome binding site characterized by IIT Madras in iGEM 2016.</p> | |
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<p class="montserrat-text uppercase">RFP (E1010)</p> | <p class="montserrat-text uppercase">RFP (E1010)</p> | ||
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| − | + | <p>Fluorescent proteins are very common reporter genes. This particular one comes from <i>Discosoma striata</i> and was engineered to be monomeric. The excitation peak is at 584 nm, and the emission peak at 607 nm.</p> | |
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Revision as of 16:37, 1 November 2017
In the lab
Parts we created
ComR (BBa_K2341000)
Protein originally found in Streptococcus thermophilius where it regulates natural competence. The protein activation and binding to specific promoters (ComR Box; BBa_K2341001) is only possible in presence of a short peptide, ComS (LPYFAGCL). Binding to the promoter will induce the downstream sequence expression.
P1655 promoter (BBa_K2341001)
Promote expression of the sequence downstream in presence of ComR (BBa_K2341000) and ComS (LPYFAGCL). Sequence containing the palindrome motif needed to bind ComR (= ComR box).
sgRNA Tyrosin (Cas9 guide) (BBa_K2341100)
RNA guide to use in combination with CRISPR/CAS9. Cut inside the tyrA gene so that deletion by homologous recombination can occur if a homologous DNA strand is also added. RNA guide under constitutive expression by BBa_J23119.
Parts we used
pBAD/araC (I0500)
The pBAD promoter is a very common inducible one that is activated by L-arabinose and repressed by araC.
Terminator (B0010)
Terminators are necessary to stop the transcription at the end of a coding sequence.
RBS (B0034)
Efficient ribosome binding site characterized by IIT Madras in iGEM 2016.
RFP (E1010)
Fluorescent proteins are very common reporter genes. This particular one comes from Discosoma striata and was engineered to be monomeric. The excitation peak is at 584 nm, and the emission peak at 607 nm.
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