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current shortcomings of acrylic biosynthesis method.How to come up with a short and efficient acrylic biosynthetic pathway to build a highly efficient acrylic acid biosynthetic factory is the key to success! This is also | current shortcomings of acrylic biosynthesis method.How to come up with a short and efficient acrylic biosynthetic pathway to build a highly efficient acrylic acid biosynthetic factory is the key to success! This is also | ||
the entry point for our project this year. | the entry point for our project this year. | ||
− | <br><br> In the previous experiments, we had further demonstrated the new function of ceaS2 enzyme, which can catalyze the production of acrylic acid with DHAP (hydroxyxy acetone phosphate) or G3P (glyceraldehyde 3-phosphate) as substrate. Because acrylic acid is not the main product of ceaS2 enzyme, the catalytic | + | <br> |
+ | <img src="https://static.igem.org/mediawiki/2017/e/e6/Butongdaixietonglu.jpeg" class="img-responsive"> | ||
+ | <br> In the previous experiments, we had further demonstrated the new function of ceaS2 enzyme, which can catalyze the production of acrylic acid with DHAP (hydroxyxy acetone phosphate) or G3P (glyceraldehyde 3-phosphate) as substrate. Because acrylic acid is not the main product of ceaS2 enzyme, the catalytic | ||
effect of wild-type ceaS2 enzyme is very weak and the yield of acrylic acid is only 1mg / L. So we | effect of wild-type ceaS2 enzyme is very weak and the yield of acrylic acid is only 1mg / L. So we | ||
carried out engineering modification of this enzyme to improve the catalytic effect of the core part. | carried out engineering modification of this enzyme to improve the catalytic effect of the core part. |
Revision as of 17:47, 1 November 2017