Line 177: | Line 177: | ||
<div class="col-md-9"> | <div class="col-md-9"> | ||
− | <h2 id="section-1" style="padding-top: 100px; margin-top: -50px;"> | + | <h2 id="section-1" style="padding-top: 100px; margin-top: -50px;">1. Core-part:the activity of rate limiting enzyme ceaS2 has been improved</h2> |
<h4> | <h4> | ||
1. Excise the agarose gel slice, transfer the gel slice into a 1.5ml microfuge tube. | 1. Excise the agarose gel slice, transfer the gel slice into a 1.5ml microfuge tube. | ||
Line 195: | Line 195: | ||
<br /> | <br /> | ||
</h4> | </h4> | ||
− | <h2 id="section-2" style="padding-top: 100px; margin-top: -50px;"> | + | <h2 id="section-2" style="padding-top: 100px; margin-top: -50px;">2.System:S. cerevisiae is more suitable for chassis cells than E. coli</h2> |
<h4>Agarose-Electrophoresis is used in order to see if the PCR product is correct and seperate DNA by the number of | <h4>Agarose-Electrophoresis is used in order to see if the PCR product is correct and seperate DNA by the number of | ||
base pairs. | base pairs. | ||
Line 203: | Line 203: | ||
<br /> | <br /> | ||
</h4> | </h4> | ||
− | <h2 id="section-3" style="padding-top: 100px; margin-top: -50px;"> | + | <h2 id="section-3" style="padding-top: 100px; margin-top: -50px;">3.Pathway:Successfully build a new acrylic acid synthesis pathway and increase acrylic acid production</h2> |
<h4> | <h4> | ||
1. Set up the reaction. | 1. Set up the reaction. | ||
Line 221: | Line 221: | ||
<h4> </h4> | <h4> </h4> | ||
<h4> </h4> | <h4> </h4> | ||
− | <h2 id="section-4" style="padding-top: 100px; margin-top: -50px;"> | + | <h2 id="section-4" style="padding-top: 100px; margin-top: -50px;">4.Product:Multi - Conditional Optimization of Acrylic Cell Factory Catalytic Reaction Process</h2> |
<h4> | <h4> | ||
for acrylic acid | for acrylic acid | ||
Line 248: | Line 248: | ||
<br /> | <br /> | ||
</h4> | </h4> | ||
− | <h2 id="section-5" style="padding-top: 100px; margin-top: -50px;"> | + | <h2 id="section-5" style="padding-top: 100px; margin-top: -50px;">5.Conclusion </h2> |
<h4>1. Pre-chill 1.5ml and 2ml microcentrifuge tubes, deionized water, 10% glycerol. Add 1ml LB to 2ml microcentrifuge | <h4>1. Pre-chill 1.5ml and 2ml microcentrifuge tubes, deionized water, 10% glycerol. Add 1ml LB to 2ml microcentrifuge | ||
tubes. | tubes. |
Revision as of 16:43, 1 November 2017