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</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/7/7b/%E9%85%B5%E6%AF%8D%E5%8E%9F%E5%A7%8B%E4%BB%A3%E8%B0%A2%E5%9B%BE.png" class="img-responsive"> |
<h4> </h4> | <h4> </h4> | ||
</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/4/47/%E9%85%B5%E6%AF%8D1_Y33-Leu-ceas2_9033.png" class="img-responsive"> |
<h4> </h4> | <h4> </h4> | ||
</div> | </div> | ||
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− | <br><br> | + | <br><br> We confirmed the proposal can make S.cerevisiae produce acrylic acid, but the |
yield is low, so we decided to optimize it. | yield is low, so we decided to optimize it. | ||
<br> First, according to GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) in E.coli, we added NOX to the pathway(the | <br> First, according to GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) in E.coli, we added NOX to the pathway(the | ||
CAT enzyme is active in S.cerevisiae). So we designed a pathway, GNDC(GlyDH-NOX -DAK-ceaS2), | CAT enzyme is active in S.cerevisiae). So we designed a pathway, GNDC(GlyDH-NOX -DAK-ceaS2), | ||
for S.cerevisiae. | for S.cerevisiae. | ||
− | <br><br> | + | |
+ | <div class="col-md-12" style="padding-top:30px"> | ||
+ | <div class="col-md-4"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/23/%E9%85%B5%E6%AF%8D3_Y33-URA-gld-DAK_10763.png" class="img-responsive"> | ||
+ | <h4> </h4> | ||
+ | </div> | ||
+ | <div class="col-md-4"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/b/b0/%E9%85%B5%E6%AF%8D%E8%B7%AF%E5%BE%84%E5%9B%BE.png" class="img-responsive"> | ||
+ | <h4> </h4> | ||
+ | </div> | ||
+ | <div class="col-md-4"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/25/%E9%85%B5%E6%AF%8D2_Y33-leu-ceas2-NOX_10513.png" class="img-responsive"> | ||
+ | <h4> </h4> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <br><br> The genes of GlyDH and DAK were constructed on the backbone of YCPlac33 plasmid with | ||
URA marker. We used the ADH1 promoter and tGPD1 terminator for GlyDH, the PGK1 promoter and the | URA marker. We used the ADH1 promoter and tGPD1 terminator for GlyDH, the PGK1 promoter and the | ||
tPFK1 terminator for DAK. NOX and ceaS2 were constructed on the backbone of the other YCPlac33 | tPFK1 terminator for DAK. NOX and ceaS2 were constructed on the backbone of the other YCPlac33 |
Revision as of 18:43, 1 November 2017