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in prokaryote and Saccharomyces cerevisiae which is the most easily manipulated chassis organism | in prokaryote and Saccharomyces cerevisiae which is the most easily manipulated chassis organism | ||
in eukaryotes. | in eukaryotes. | ||
+ | |||
+ | <center><img src="https://static.igem.org/mediawiki/2017/8/85/System.png" class="img-responsive"></center> | ||
+ | |||
+ | |||
<br><br> The GDC (GlyDH-DAK-ceaS2) pathway was designed in order to improve the ability of chassis cells | <br><br> The GDC (GlyDH-DAK-ceaS2) pathway was designed in order to improve the ability of chassis cells | ||
to convert glycerol to DHAP and finally synthesize acrylic acid. We also added NOX (NADH dehydrogenase) | to convert glycerol to DHAP and finally synthesize acrylic acid. We also added NOX (NADH dehydrogenase) | ||
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can increase the carbon flow of DHAP and G3P, thereby contributing to the increase in the production | can increase the carbon flow of DHAP and G3P, thereby contributing to the increase in the production | ||
of acrylic acid. | of acrylic acid. | ||
+ | |||
+ | <div class="col-md-12" style="padding-top:30px"> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/1/10/%E5%A4%A7%E8%82%A0%E8%B7%AF%E5%BE%84%E5%9B%BE.png" class="img-responsive"> | ||
+ | <h4> </h4> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/b/b0/%E9%85%B5%E6%AF%8D%E8%B7%AF%E5%BE%84%E5%9B%BE.png" class="img-responsive"> | ||
+ | <h4> </h4> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
<br><br> We used whole cell catalysis to carry out the initial acrylic acid synthesis "fermentation" process. | <br><br> We used whole cell catalysis to carry out the initial acrylic acid synthesis "fermentation" process. | ||
The advantage of whole cell catalysis is that the intracellular complete multi-enzyme system can | The advantage of whole cell catalysis is that the intracellular complete multi-enzyme system can | ||
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<br><br> We optimized the reaction process, selected the carbon source, buffer, temperature, pH, reaction | <br><br> We optimized the reaction process, selected the carbon source, buffer, temperature, pH, reaction | ||
time and other conditions to optimize the production process of the cell factory. | time and other conditions to optimize the production process of the cell factory. | ||
− | <br><br> We also made Hardware<a href="https://2017.igem.org/Team:NPU-China/Hardware">(Click Here)</a> to simulate the industrial production process of acrylic acid!</h4> | + | <center><img src="https://static.igem.org/mediawiki/2017/6/67/Production2.png" class="img-responsive"></center> |
+ | <br><br> We also made Hardware<a href="https://2017.igem.org/Team:NPU-China/Hardware">(Click Here)</a> to simulate the industrial production process of acrylic acid! | ||
+ | |||
+ | </h4> | ||
</div> | </div> |
Revision as of 21:02, 1 November 2017