Shuimoliuyun (Talk | contribs) |
Shuimoliuyun (Talk | contribs) |
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</h4><br> | </h4><br> | ||
<h4>4.pSBC3-TEF2+NOX+tRPS2(BBa_K2347008)<br><h4> | <h4>4.pSBC3-TEF2+NOX+tRPS2(BBa_K2347008)<br><h4> | ||
− | <h4>In this part, the promoter of NOX is TEF2 promoter, and the terminator is tRPS2 terminator. TEF2 promoter is a kind of promoter which has strong expression and tRPS2 terminator is a terminator with rather strong expression ability. So they can both increase the expression of genes. At the same time they are constitutive promoters and terminators, which have the ability to integrate gene fragment, NOX, into the chromosome of yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with part pSBC3-pTDH3+ceas2+ | + | <h4>In this part, the promoter of NOX is TEF2 promoter, and the terminator is tRPS2 terminator. TEF2 promoter is a kind of promoter which has strong expression and tRPS2 terminator is a terminator with rather strong expression ability. So they can both increase the expression of genes. At the same time they are constitutive promoters and terminators, which have the ability to integrate gene fragment, NOX, into the chromosome of yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with part pSBC3-pTDH3+ceas2+tPFK1 and the YCplac33 plasmid vector to form intact plasmid with LEU deficient. |
</h4><br> | </h4><br> | ||
Revision as of 22:02, 1 November 2017