Line 253: | Line 253: | ||
</div> | </div> | ||
− | <center> <h4>pSBC3-ADH1+gld+ | + | <center> <h4>pSBC3-ADH1+gld+tGPD1 & pSBC3-PGK1+DAK+TPFK1<br><h4></center> |
<h4>The enzyme GlyDH (gld) and DAK can be efficiently expressed in the yeast cell, and the gene of the | <h4>The enzyme GlyDH (gld) and DAK can be efficiently expressed in the yeast cell, and the gene of the | ||
enzyme GlyDH (gld) and DAK are integrated into the chromosome. In this pathway, GlyDH (Glycerol | enzyme GlyDH (gld) and DAK are integrated into the chromosome. In this pathway, GlyDH (Glycerol | ||
Line 263: | Line 263: | ||
</h4> | </h4> | ||
− | + | <h4>3.pSBC3-pTDH3+ceas2+tPFK1<br><h4> | |
<h4>In this part, the promoter of ceaS2 is pTDH3 promoter, and the terminator is tPFK1 terminator. pTDH3 | <h4>In this part, the promoter of ceaS2 is pTDH3 promoter, and the terminator is tPFK1 terminator. pTDH3 | ||
promoter is a kind of promoter which has strong expression and tPFK1 terminator is a terminator with | promoter is a kind of promoter which has strong expression and tPFK1 terminator is a terminator with | ||
Line 271: | Line 271: | ||
with the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | with the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | ||
</h4><br> | </h4><br> | ||
+ | <h4> | ||
+ | <div class="col-md-12" style="padding-top:30px"> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/6/6c/PSB1C3-pTDH3%2Bceas2%2BtPFK1.png" class="img-responsive"> | ||
+ | <h4> </h4> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/0/06/YCplac-pTDH3-ceaS2-tPFK1.png" class="img-responsive"> | ||
+ | <h4> </h4> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <h4>In the yeast cells, the enzyme ceaS2 can be efficiently expressed and the gene of ceas2 is integrated into | ||
+ | the chromosome. With the help of TPP (Thiamine pyrophosphate) and magnesium ions, ceaS2 can | ||
+ | catalyze the production of acrylic acid with DHAP (dihydroxy acetone phosphate) and G3P | ||
+ | (glyceraldehyde 3-phosphate ) as substrate. </h4><br> | ||
+ | |||
+ | </h4> | ||
+ | |||
+ | |||
<h4>4.pSBC3-TEF2+NOX+tRPS2<br><h4> | <h4>4.pSBC3-TEF2+NOX+tRPS2<br><h4> | ||
<h4>In this part, the promoter of NOX is TEF2 promoter, and the terminator is tRPS2 terminator. TEF2 promoter is | <h4>In this part, the promoter of NOX is TEF2 promoter, and the terminator is tRPS2 terminator. TEF2 promoter is | ||
Line 279: | Line 302: | ||
pSBC3-pTDH3+ceas2+tPFK1and the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | pSBC3-pTDH3+ceas2+tPFK1and the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | ||
</h4><br> | </h4><br> | ||
− | + | <h4> <div class="col-md-12" style="padding-top:30px"> | |
− | < | + | <div class="col-md-6"> |
− | + | <img src="https://static.igem.org/mediawiki/2017/c/c4/PSB1C3-TEF2-NOX-tRPS2.png" class="img-responsive"> | |
− | + | <h4> </h4> | |
− | + | </div> | |
− | + | <div class="col-md-6"> | |
− | + | <img src="https://static.igem.org/mediawiki/2017/8/86/YCplac33-Leu-NOX-ceaS2.png" class="img-responsive"> | |
− | < | + | <h4> </h4> |
− | + | ||
− | + | </div> | |
− | + | </div> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | < | + | |
− | < | + | |
<h4>It can efficiently express the enzyme NOX in yeast and integrate NOX into the chromosome. Since GlyDH | <h4>It can efficiently express the enzyme NOX in yeast and integrate NOX into the chromosome. Since GlyDH | ||
is an NAD+ -dependent enzyme, NOX and CAT(which already exists in yeast) provide the required | is an NAD+ -dependent enzyme, NOX and CAT(which already exists in yeast) provide the required | ||
reduction force for GLYDH through the two layers of substrate level cycle. | reduction force for GLYDH through the two layers of substrate level cycle. | ||
</h4><br> | </h4><br> | ||
+ | </h4> | ||
</div> | </div> | ||
Revision as of 22:38, 1 November 2017