Difference between revisions of "Team:UCopenhagen/Protocols"
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To make sure we perform our experiments consistently, and that they can be reproduced by other scientists, we have written protocols for the experiments we have performed across the three subprojects. We have collected them all together below: | To make sure we perform our experiments consistently, and that they can be reproduced by other scientists, we have written protocols for the experiments we have performed across the three subprojects. We have collected them all together below: | ||
<br><br> | <br><br> | ||
| − | <strong>General ( | + | <strong>General (Used across subprojects)</strong> |
<ul style="text-align:left; color:white;"> | <ul style="text-align:left; color:white;"> | ||
| − | <li> <a href="https://static.igem.org/mediawiki/2017/2/2e/Protocol1_UCopenhagen.pdf">PCR</span></a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2017/2/2e/Protocol1_UCopenhagen.pdf">Protocol for PCR</span></a></li> |
| − | <li> | + | <li><a href="https://static.igem.org/mediawiki/2017/c/cd/GelElectrophoresis_UCopenhagen.pdf">Protocol for gel electrophoresis</span></a></li> |
| − | <li> Protocol | + | <li><a href="https://static.igem.org/mediawiki/2017/c/c6/Protocol3_UCopenhagen.pdf">Protocol for plasmid purification</span></a></li> |
| − | <li> Protocol | + | <li><a href="https://static.igem.org/mediawiki/2017/9/93/DNA_Purification_gel_extraction.pdf">Protocol for DNA purification by gel extraction</span></a></li> |
| + | <li><a href="https://static.igem.org/mediawiki/2017/9/94/Protocol8_UCopenhagen.pdf">Protocol for Mix&Go Transformation</span></a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/6/62/Protocol14_UCopenhagen.pdf">Protocol for LB media</span></a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/b/b0/Protocol16_UCopenhagen.pdf">Protocol for Colony PCR</span></a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/9/9f/Protocol17_UCopenhagen.pdf">Protocol for DNA sequencing</span></a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/f/fe/Growth_curve_general.pdf">Protocol for growth curves and OD<sub>600</sub> measurements</span></a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/2/25/Protocol7_UCopenhagen.pdf">Protocol for USER ligation</span></a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/9/96/USER_linearization.pdf">Protocol for USER linearization</span></a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/3/36/Blunt_ligation.pdf">Protocol for blunt ligation</span></a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/a/ab/Protocol13_UCopenhagen.pdf">Protocol for Restriction digestion</span></a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/f/fa/Transformation_into_BL21.pdf">Protocol for transformation into BL21</span></a></li> | ||
</ul> | </ul> | ||
</p> | </p> | ||
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<strong>Interdependency</strong> | <strong>Interdependency</strong> | ||
<ul style="text-align:left; color:white;"> | <ul style="text-align:left; color:white;"> | ||
| − | <li> | + | <li><a href="https://static.igem.org/mediawiki/2017/f/f6/Protocol18_UCopenhagen.pdf">Protocol for YNB media</span></a></li> |
| − | < | + | <li><a href="https://static.igem.org/mediawiki/2017/0/0e/Protocol19_UCopenhagen.pdf">Protocol for serial growth experiments</span></a></li> |
| − | <li> | + | <li><a href="https://static.igem.org/mediawiki/2017/b/b8/Protocol20_UCopenhagen.pdf">Protocol for HPLC-MS measurements of tryptophan concentration</span></a></li> |
| − | <li> | + | |
</ul> | </ul> | ||
</p> | </p> | ||
| Line 55: | Line 64: | ||
<strong>Number control</strong> | <strong>Number control</strong> | ||
<ul style="text-align:left; color:white;"> | <ul style="text-align:left; color:white;"> | ||
| − | <li> | + | <li><a href="https://static.igem.org/mediawiki/2017/0/07/GRNA_seed_sequence_insertion_via_PCR.pdf">Protocol for gRNA seed sequence insertion</span></a></li> |
| − | < | + | <li><a href="https://static.igem.org/mediawiki/2017/d/dd/Heat_shock_transformation.pdf">Protocol for Heat-shock transformation</span></a></li> |
| − | <li> | + | <li><a href="https://static.igem.org/mediawiki/2017/2/26/Refined_growth_curve.pdf">Protocol for refined growth curve</span></a></li> |
| − | <li> | + | |
</ul> | </ul> | ||
</p> | </p> | ||
| Line 65: | Line 73: | ||
<strong>Protein Import</strong> | <strong>Protein Import</strong> | ||
<ul style="text-align:left; color:white;"> | <ul style="text-align:left; color:white;"> | ||
| − | <li> | + | <li><a href="https://static.igem.org/mediawiki/2017/8/88/Protein_extractions_and_purification.pdf">Protocol for protein extraction and purification</span></a></li> |
| − | <li> | + | <li><a href="https://static.igem.org/mediawiki/2017/d/d3/Western_Blotting.pdf">Protocol for Western Blotting</span></a></li> |
| − | <li> | + | <li><a href="https://static.igem.org/mediawiki/2017/b/b3/SDS-Page.pdf">Protocol for SDS page</span></a></li> |
| − | <li> | + | <li><a href="https://static.igem.org/mediawiki/2017/0/0e/Protocol29_UCopenhagen.pdf">Protocol for fluorescence microscopy</span></a></li> |
</ul> | </ul> | ||
</p> | </p> | ||
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<li> | <li> | ||
<a class="page-scroll" href="#Top">Introduction</a> | <a class="page-scroll" href="#Top">Introduction</a> | ||
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</li> | </li> | ||
Revision as of 23:38, 1 November 2017
To make sure we perform our experiments consistently, and that they can be reproduced by other scientists, we have written protocols for the experiments we have performed across the three subprojects. We have collected them all together below:
General (Used across subprojects)
- Protocol for PCR
- Protocol for gel electrophoresis
- Protocol for plasmid purification
- Protocol for DNA purification by gel extraction
- Protocol for Mix&Go Transformation
- Protocol for LB media
- Protocol for Colony PCR
- Protocol for DNA sequencing
- Protocol for growth curves and OD600 measurements
- Protocol for USER ligation
- Protocol for USER linearization
- Protocol for blunt ligation
- Protocol for Restriction digestion
- Protocol for transformation into BL21
Interdependency
- Protocol for YNB media
- Protocol for serial growth experiments
- Protocol for HPLC-MS measurements of tryptophan concentration
Number control
- Protocol for gRNA seed sequence insertion
- Protocol for Heat-shock transformation
- Protocol for refined growth curve
Protein Import
