m (by LC) |
m (by LC) |
||
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<div class="block main-text main-text"> | <div class="block main-text main-text"> | ||
<h class="highlight-title dark-blue" id="Antigen"></br></br>How pSV40-ZF43-8;8*works </h> | <h class="highlight-title dark-blue" id="Antigen"></br></br>How pSV40-ZF43-8;8*works </h> | ||
− | <p>The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ | + | <p>The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats. |
</p> | </p> | ||
Line 77: | Line 77: | ||
<div class="block main-text main-text"> | <div class="block main-text main-text"> | ||
<h class="highlight-title dark-blue" id="Antigen"></br></br>The advantages of pSV40-ZF43-8;8*</h> | <h class="highlight-title dark-blue" id="Antigen"></br></br>The advantages of pSV40-ZF43-8;8*</h> | ||
− | <p>Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The | + | <p>Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basal expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state. |
</p> | </p> | ||
<p> | <p> | ||
− | The | + | The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats. |
</p> | </p> | ||
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<h class="highlight-title dark-blue" id="Antigen"></br></br>See More</h> | <h class="highlight-title dark-blue" id="Antigen"></br></br>See More</h> | ||
<p> | <p> | ||
− | Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446028">(BBa_K2446028)</a> on the Registry of Standard Biological Parts website. | + | Integrated information about surEGFP posted on <a href="https://2017.igem.org/Team:Fudan/Demonstrate">Demonstrate</a> page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446028">(BBa_K2446028)</a> on the Registry of Standard Biological Parts website. |
</p> | </p> | ||
<p> | <p> |
Revision as of 23:32, 1 November 2017
This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).
The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.
Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basal expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.
The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.
Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website.
We listed all the composite parts we constructed this year here.