Difference between revisions of "Team:Fudan/Composite Part"

Revision as of 23:38, 1 November 2017

Composite part – SynPro(S)-ZF43-8;8*

For this year’s iGEM competition, our favorite basic part is SynPro(S)-43-8;8*
(BBa_K2446028).

This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).

How SynPro(S)-ZF43-8;8*works

The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.

The advantages of pSV40-ZF43-8;8*

Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basal expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.

The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.

Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website.

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-   <h class="highlight-title dark-blue" id="Antigen"></br></br>How pSV40-ZF43-8;8*works </h>
+   <h class="highlight-title dark-blue" id="Antigen"></br></br>How SynPro(S)-ZF43-8;8*works </h>
    <p>The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.
 </p>