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</div> | </div> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>SV40_8_ZF_42-10 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446025”>(BBa_K2446025)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_2_ZF_43-8 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446026”>(BBa_K2446026)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_4_ZF_43-8 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446027”>(BBa_K2446027)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_8_ZF_54-8 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446029”>(BBa_K2446029)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_8_ZF_21-16 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446030”>(BBa_K2446030)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_8_ZF54-8/43-8 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446031”>(BBa_K2446031)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_4_ZFHD1 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446032”>(BBa_K2446032)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_2_PIP promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446033”>(BBa_K2446033)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_4_PIP promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446034”>(BBa_K2446034)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_8_PIP promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446035”>(BBa_K2446035)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SV40_UAS promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446036”>(BBa_K2446036)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CMV_8_ZF_42-10 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446018”>(BBa_K2446018)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CMV_8_ZF_43-8 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446019”>(BBa_K2446019)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CMV_8_ZF_54-8 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446020”>(BBa_K2446020)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CMV_8_ZF54-8/43-8 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446021”>(BBa_K2446021)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CMV_4_ZFHD1 promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446022”>(BBa_K2446022)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CMV_4_PIP promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446023”>(BBa_K2446023)</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CMV_8_PIP promoter</td> | ||
+ | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446024”>(BBa_K2446024)</a></td> | ||
+ | </tr> | ||
+ | </table> | ||
Revision as of 00:46, 2 November 2017
This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).
The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.
Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basal expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.
The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.
Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website.
SV40_8_ZF_42-10 promoter | (BBa_K2446025) |
SV40_2_ZF_43-8 promoter | (BBa_K2446026) |
SV40_4_ZF_43-8 promoter | (BBa_K2446027) |
SV40_8_ZF_54-8 promoter | (BBa_K2446029) |
SV40_8_ZF_21-16 promoter | (BBa_K2446030) |
SV40_8_ZF54-8/43-8 promoter | (BBa_K2446031) |
SV40_4_ZFHD1 promoter | (BBa_K2446032) |
SV40_2_PIP promoter | (BBa_K2446033) |
SV40_4_PIP promoter | (BBa_K2446034) |
SV40_8_PIP promoter | (BBa_K2446035) |
SV40_UAS promoter | (BBa_K2446036) |
CMV_8_ZF_42-10 promoter | (BBa_K2446018) |
CMV_8_ZF_43-8 promoter | (BBa_K2446019) |
CMV_8_ZF_54-8 promoter | (BBa_K2446020) |
CMV_8_ZF54-8/43-8 promoter | (BBa_K2446021) |
CMV_4_ZFHD1 promoter | (BBa_K2446022) |
CMV_4_PIP promoter | (BBa_K2446023) |
CMV_8_PIP promoter | (BBa_K2446024) |