Difference between revisions of "Team:Arizona State/Results"

 
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<p>The specific senders that were chosen for the induction tests were selected because previous research showed that they have either a very low or very high rate of GFP induction when used in a single sender/ receiver circuit. In other words, the chosen senders tend to either work very well or not very well at all. More data is needed on how well these senders express the gene when used in combination with another. By combining two senders at a time, sometimes with senders that have shown to induce a high GFP expression and sometimes with senders that have shown a weak induction, the goal is to see if there is any increase or decrease the GFP expression on demand. The controls used for the experiment were single sender inductions on the same plate as the combinations, the use of blank wells (LB AMP 100%), a positive GFP control, and a negative control with negative receiver cells and negative sender supernatant.</p>
 
<p>The specific senders that were chosen for the induction tests were selected because previous research showed that they have either a very low or very high rate of GFP induction when used in a single sender/ receiver circuit. In other words, the chosen senders tend to either work very well or not very well at all. More data is needed on how well these senders express the gene when used in combination with another. By combining two senders at a time, sometimes with senders that have shown to induce a high GFP expression and sometimes with senders that have shown a weak induction, the goal is to see if there is any increase or decrease the GFP expression on demand. The controls used for the experiment were single sender inductions on the same plate as the combinations, the use of blank wells (LB AMP 100%), a positive GFP control, and a negative control with negative receiver cells and negative sender supernatant.</p>
  
<center><img src=" https://static.igem.org/mediawiki/2017/0/0d/Results_Summ.png"></center>
 
  
 
<p><ins><b>This next section of results is for the tests done with the next receiver, LasR. </b></ins></p>
 
<p><ins><b>This next section of results is for the tests done with the next receiver, LasR. </b></ins></p>

Latest revision as of 03:36, 2 November 2017