Difference between revisions of "Team:Nanjing-China"

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<title>Team:Nanjing-China - 2017.igem.org</title>
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<div id="HOME"><img src="https://static.igem.org/mediawiki/2017/e/e1/T-Nanjing-China-signal.png" width="100%">
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<div class="sub">
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  <ul><li><a href="#https://2017.igem.org/Team:Nanjing-China/Introduction">Introduction</a></ul></li></div>
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      <ul>
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    <li><a href="#abstract">Abstract</a></li>
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  <li><a href="#introduction">Introduction</a></li>
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  </ul>
 
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<div class="container" align="center">
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<div id="menu">
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<div class="column full_size" >
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  <ul>
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<h1> Welcome to iGEM 2017! </h1>
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  <li><a href="https://2017.igem.org/Team:Nanjing-China">HOME</a>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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    <ul>
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</div>  
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        <li><a href="https://2017.igem.org/Team:Nanjing-China">Introduction</a></li>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Background">Background</a></li>
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<div class="clear"></div>
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</ul>
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    </li>
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<div class="column half_size" >
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    <li><a href="https://2017.igem.org/Team:Nanjing-China/CH2O">PARTS</a>
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<h5>Before you start: </h5>
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    <ul>
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<p> Please read the following pages:</p>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/CH2O">CH<sub>2</sub>o</a></li>
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<ul>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/H2">H<sub>2</sub></a></li>
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<li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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          <li><a href="https://2017.igem.org/Team:Nanjing-China/H2S">H<sub>2</sub>S</a></li>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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</ul>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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    </li>
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</ul>
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    <li><a href="https://2017.igem.org/Team:Nanjing-China/Sliver">JUDGE</a>
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    <ul>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/Sliver">Sliver</a></li>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Gold">Gold</a></li>
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</ul>
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      </li>
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    <li><a href="https://2017.igem.org/Team:Nanjing-China/Design">PROJECT</a>
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    <ul>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/Design">Design</a></li>
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          <li><a href="https://2017.igem.org/Team:Nanjing-China/Experiments">Experiments</a></li>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/Notebook">Notebook</a></li>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Contribution">Contribution</a></li>
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          <li><a href="https://2017.igem.org/Team:Nanjing-China/Results">Results</a></li>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Improvement">Improvement</a></li>
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</ul>
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        </li>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/Introduction">TEAM</a>
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    <ul>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/Introduction">Introduction</a></li>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Members">Members</a></li>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/Collaboration">Collaboration</a></li>
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</ul>
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    </li>
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    <li><a href="https://2017.igem.org/Team:Nanjing-China/OTHERS">OTHERS</a>
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    <ul>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/Safety">Safety</a></li>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Model">Model</a></li>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/HP">HP</a></li>
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</ul>
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    </li>
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      </ul>
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  </div>
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  </div>
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    <div class="contain">
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<div align="center">
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  <div id="abstract">
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      <img src="https://static.igem.org/mediawiki/2017/3/3b/T-Nanjing-China-t-abstract.png" width="60%" /><br /></div>
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        <div align="left">
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          <blockquote>
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            <blockquote>
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              <blockquote>
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                <p><img src="https://static.igem.org/mediawiki/2017/f/f5/T-Nanjing-China-point.png" width="30%"/>
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                </p>
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              </blockquote>
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            </blockquote>
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          </blockquote>
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        </div>
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      <div class="word">
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    <h2>What are biosensors?</h2>
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      <p>- Devices that use a biological component such as enzymes, antibodies, sub-cellular components,
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        and microorganisms to obtain information.
 +
      </p>
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<h2>What are biosensors for?</h2>
 +
      <p> - They are designed to detect small amounts of chemical, or to report physiological and biochemical characteristics of a sample.</p></td>
 +
<h2>What’s the difference between biosensors and whole-cell sensors?</h2>
 +
    <p>-A whole-cell sensing system depends on the integrity of a cell or a microorganism.<br />
 +
      -It includes a series of biochemical reactions inside the cell.</p>
 +
          </div>
 +
    <p><img src="https://static.igem.org/mediawiki/2017/3/35/T-Nanjing-China-cartoon.png" width="95%" /></p>
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      </div>
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  <div id="introduction">
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    <div align="center"><img src="https://static.igem.org/mediawiki/2017/9/9a/T-Nanjing-China-t-introduction.png" width="60%" />
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    </div>
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    <div align="right">
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        <blockquote>
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          <blockquote>
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            <blockquote>
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              <blockquote>
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                <p><img src="https://static.igem.org/mediawiki/2017/f/f5/T-Nanjing-China-point.png" width="35%" />
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                </p>
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              </blockquote>
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            </blockquote>
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          </blockquote>
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        </blockquote>
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    </div>
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    <div align="center">
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    <div class="word">
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      <p>Formaldehyde, hydrogen sulfide and hydrogen  are three common gases that can pose great danger to our lives. Formaldehyde and  hydrogen sulfide are venomous substances released from decoration materials and  decayed remains, while formaldehyde is known to be human carcinogen. Hydrogen  is widely used in industry and laboratories, which is a potential safety threat  to factory and lab crews owing to its flammability and explosion hazard. However,  traditional methods for detecting these gases are mainly based on irreversible redox  reactions in complicated and expensive devices with short life spans, whereas it  has been reported that whole-cell bacterial biosensors have major advantages  over traditional analyses with regard to specificity, sensitivity and  portability.</p>
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            <p>Our team attempts to develop a whole-cell  system detecting formaldehyde, hydrogen sulfide and hydrogen. We have managed  to transfer gene clusters encoding gas-specific translational suppressors or  activator into E. coli to respond to different gas molecules by up regulation  of specific downstream genes. In order to visualize the gases&rsquo; existence and  concentration, genes coding for fluorescent proteins are also inserted in the  expression system after the promoter influenced by the suppressor or activator.  At present, results have shown sensibility at a µM level.</p>
 +
            <p>As long as proper containers are designed,  we will be able to construct portable whole-cell gas sensors by which users can  perform instant on-site monitoring with higher sensitivity, accuracy and  stability but lower costs. </p>
 +
            </div>
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    </div>
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  </div>
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    </div>
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  <div class="footer">
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    <div align="center">
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      <div class="f">
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        <div class="f-word"><p><strong><u>Address</u></strong></p>
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            <p>Life Science Department<br />
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              #163 Xianlin Blvd, Qixia District<br />
 +
              Nanjing University<br />
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              Nanjing, Jiangsu Province<br />
 +
              P.R. of China<br />
 +
              Zip: 210046
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            </p>
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            <p><strong><u>Email</u></strong></p>
 +
            <p>nanjing_china@163.com</p>
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            <p><strong><u>Social media</u></strong></p>
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        </div>
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        <div class="f-img">
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          <div align="center"><img src="https://static.igem.org/mediawiki/2017/0/04/T-Nanjing-China-%E5%8D%97%E4%BA%AC%E5%A4%A7%E5%AD%A6.png" name="img1" width="135" height="195" id="img1" onmouseover="MM_changeProp('img1','','width','178px','IMG');MM_changeProp('img1','','height','228px','IMG')" onmouseout="MM_changeProp('img1','','width','135px','IMG');MM_changeProp('img1','','height','195px','IMG')" /></div>
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        </div>
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          <div class="f-img">
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            <div align="right"><img src=" https://static.igem.org/mediawiki/2017/d/d4/T-Nanjing-China-%E5%9B%BE%E7%89%873.png
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" name="img2" width="135" height="195" id="img2"  onmouseover="MM_changeProp('img2','','width','176px','IMG');MM_changeProp('img2','','height','228px','IMG')" onmouseout="MM_changeProp('img2','','width','135px','IMG');MM_changeProp('img2','','height','195px','IMG')" /></div>
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          </div>
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          <div class="f-img">
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            <div align="center"><img src="https://static.igem.org/mediawiki/2017/3/37/T-Nanjing-China-s1.png" name="img3" width="140" height="139" id="img3"  onmouseover="MM_changeProp('img3','','width','179px','IMG');MM_changeProp('img3','','height','179px','IMG')" onmouseout="MM_changeProp('img3','','width','140px','IMG');MM_changeProp('img3','','height','139px','IMG')" align="absmiddle" /></div>
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          </div>
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          <div class="f-img">
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            <div align="right"><img src="https://static.igem.org/mediawiki/2017/d/dc/T-Nanjing-China-%E5%9B%BE%E7%89%872.png" name="img4" width="143" height="139" id="img4"  onmouseover="MM_changeProp('img4','','width','180px','IMG');MM_changeProp('img4','','height','179px','IMG')" onmouseout="MM_changeProp('img4','','width','143px','IMG');MM_changeProp('img4','','height','139px','IMG')" /></div>
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      <img src="https://static.igem.org/mediawiki/2017/9/91/T-Nanjing-China-foot.png" width="100%"/>
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</body>
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<div class="column half_size" >
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<div class="highlight">
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<div class="column half_size" >
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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Revision as of 14:54, 17 October 2017

Team:Nanjing-China - 2017.igem.org


What are biosensors?

- Devices that use a biological component such as enzymes, antibodies, sub-cellular components, and microorganisms to obtain information.

What are biosensors for?

- They are designed to detect small amounts of chemical, or to report physiological and biochemical characteristics of a sample.

What’s the difference between biosensors and whole-cell sensors?

-A whole-cell sensing system depends on the integrity of a cell or a microorganism.
-It includes a series of biochemical reactions inside the cell.

Formaldehyde, hydrogen sulfide and hydrogen are three common gases that can pose great danger to our lives. Formaldehyde and hydrogen sulfide are venomous substances released from decoration materials and decayed remains, while formaldehyde is known to be human carcinogen. Hydrogen is widely used in industry and laboratories, which is a potential safety threat to factory and lab crews owing to its flammability and explosion hazard. However, traditional methods for detecting these gases are mainly based on irreversible redox reactions in complicated and expensive devices with short life spans, whereas it has been reported that whole-cell bacterial biosensors have major advantages over traditional analyses with regard to specificity, sensitivity and portability.

Our team attempts to develop a whole-cell system detecting formaldehyde, hydrogen sulfide and hydrogen. We have managed to transfer gene clusters encoding gas-specific translational suppressors or activator into E. coli to respond to different gas molecules by up regulation of specific downstream genes. In order to visualize the gases’ existence and concentration, genes coding for fluorescent proteins are also inserted in the expression system after the promoter influenced by the suppressor or activator. At present, results have shown sensibility at a µM level.

As long as proper containers are designed, we will be able to construct portable whole-cell gas sensors by which users can perform instant on-site monitoring with higher sensitivity, accuracy and stability but lower costs.

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

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