Difference between revisions of "Team:Nanjing-China/Parts"

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<title>Team:Nanjing-China - 2017.igem.org</title>
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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    <div align="center">
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    <div id="HOME">
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<div class="sub">
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  <ul><li><a href="#https://2017.igem.org/Team:Nanjing-China/Introduction">Introduction</a></ul></li></div>
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      <ul>
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    <li><a href="#h2">BBa_K2257000</a></li>
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  <li><a href="#h2s">BBa_K2257006</a></li>
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            <li><a href="#ch2o">BBa_K2257007</a></li>
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  </ul>
 
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<h5>Note</h5>
 
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
 
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<h5>Adding parts to the registry</h5>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
 
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<div id="menu">
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    <ul>
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  <li><a href="https://2017.igem.org/Team:Nanjing-China">HOME</a>
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    <ul>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China">Introduction</a></li>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Background">Background</a></li>
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<p>The information needed to initially create a part on the Registry is:</p>
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</ul>
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<ul>
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    </li>
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<li>Part Name</li>
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    <li><a href="https://2017.igem.org/Team:Nanjing-China/Parts">PARTS</a>
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<li>Part type</li>
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    <li><a href="https://2017.igem.org/Team:Nanjing-China/Sliver">JUDGE</a>
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<li>Creator</li>
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    <ul>
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<li>Sequence</li>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/Sliver">Sliver</a></li>
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<li>Short Description (60 characters on what the DNA does)</li>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Gold">Gold</a></li>
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<li>Long Description (Longer description of what the DNA does)</li>
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</ul>
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<li>Design considerations</li>
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      </li>
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</ul>
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    <li><a href="https://2017.igem.org/Team:Nanjing-China/Design">PROJECT</a>
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    <ul>
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<p>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/Design">Design</a></li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/Notebook">Notebook</a></li>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/Results">Results</a></li>
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</div>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Improvement">Improvement</a></li>
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</ul>
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<div class="column half_size">
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        </li>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/TEAM/Introduction">TEAM</a>
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<h5>Inspiration</h5>
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    <ul>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/TEAM/Introduction">Introduction</a></li>
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Members">Members</a></li>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
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<ul>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></li>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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</ul>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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    </li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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    <li><a href="https://2017.igem.org/Team:Nanjing-China/HP/Silver">OTHERS</a>
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    <ul>
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</div>
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        <li><a href="https://2017.igem.org/Team:Nanjing-China/HP/Silver">HP-Silver</a></li>
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                <li><a href="https://2017.igem.org/Team:Nanjing-China/HP/Gold_Integrated">HP-Gold</a></li>
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<div class="column full_size">
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            <li><a href="https://2017.igem.org/Team:Nanjing-China/Model">Model</a></li>
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<h5>Part Table </h5>
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</ul>
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    </li>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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      </ul>
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  <div id="h2"><div class="p-t" align="left"><img src="https://static.igem.org/mediawiki/2017/0/07/T-Nanjing-China-h2-3.png" width="30%" /></div>
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<groupparts>iGEM17 Nanjing-China</groupparts>
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      <div class="word" style="background-image:url(https://static.igem.org/mediawiki/2017/0/09/T-Nanjing-China-parts-b1.png)">
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      <h1>H<sub>2</sub>: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2257000">BBa_K2257000</a></h1>
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      <p></p>
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      <p></p>
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      <p>SQR is a NAD(P)/FAD-dependent  oxidoreductase, which oxidizes S2- to zero-valent S0.  SqrR, encoded by <em>sqrR</em>, is a  constitutively expressed suppressor protein of <em>sqr</em> promotor. RFP is the indicator. When there is no H2S  in the environment, SqrR combines with the <em>sqr</em> promotor and keeps the expression of both the enzyme and RFP at a low level. While  in the case where H2S exists, it will be oxidized by the small  amount of expressed enzyme, and the product will interact with SqrR, forming a  tri- or tetrasulfide cross-links linking C41 and C107 on the same subunit of  SqrR. The change in the structure of SqrR makes it fall off from the <em>sqr</em> promotor and thus the expression of  SQR and RFP turns on. The part can be used to detect hydrogen sulfide at a μmol level.</p>
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      <div style=" position:relative; top:-80px; left:-100px;" >
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        <img src="https://static.igem.org/mediawiki/2017/8/87/T-Nanjing-China-chart-cell-1.png" width="200" />
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      </div>
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      <div id="h2s"><div class="p-t" align="right"><img src="https://static.igem.org/mediawiki/2017/4/4d/T-Nanjing-China-h2s-3.png" width="30%" /></div>
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<div class="word" style="background-image:url(https://static.igem.org/mediawiki/2017/7/7a/T-Nanjing-China-parts-b2.png)">
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        <div align="left">
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        <h1>H<sub>2</sub>S: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2257006">BBa_K2257006</a></h1>
 +
        <p>SQR is a NAD(P)/FAD-dependent  oxidoreductase, which oxidizes S2- to zero-valent S0.  SqrR, encoded by <em>sqrR</em>, is a  constitutively expressed suppressor protein of <em>sqr</em> promotor. RFP is the indicator. When there is no H2S  in the environment, SqrR combines with the <em>sqr</em> promotor and keeps the expression of both the enzyme and RFP at a low level. While  in the case where H2S exists, it will be oxidized by the small  amount of expressed enzyme, and the product will interact with SqrR, forming a  tri- or tetrasulfide cross-links linking C41 and C107 on the same subunit of  SqrR. The change in the structure of SqrR makes it fall off from the <em>sqr</em> promotor and thus the expression of  SQR and RFP turns on. The part can be used to detect hydrogen sulfide at a μmol level.</p>
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</div>
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        </div>
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        <div style=" position:relative; top:-180px; left:100px; alignment-adjust:central; ">
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        <img src="https://static.igem.org/mediawiki/2017/3/3e/T-Nanjing-China-cell-r.png" width="300" />
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        </div>
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        </div>
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      <div id="ch2o">
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      <div class="p-t" align="left"><img src="https://static.igem.org/mediawiki/2017/a/a1/T-Nanjing-China-ch2o-3.png" width="30%" /></div>
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      <div class="word" style="background-image:url(https://static.igem.org/mediawiki/2017/1/11/T-Nanjing-China-parts-b3.png)">
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      <div align="left"><h1>CH<sub>2</sub>O: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2257007">BBa_K2257007</a></h1>
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      <p></p>
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      </div>
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      </div>
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      <div style=" position:relative; top:-100px; left:-30px; " align="left">
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        <img src="https://static.igem.org/mediawiki/2017/9/93/T-Nanjing-China-cell-b.png" width="130" />
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      </div>
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      </div>
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  </div>
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  <div class="footer">
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    <div align="center">
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      <div class="f">
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        <div class="f-word"><p><strong><u>Address</u></strong></p>
 +
            <p>Life Science Department<br />
 +
              #163 Xianlin Blvd, Qixia District<br />
 +
              Nanjing University<br />
 +
              Nanjing, Jiangsu Province<br />
 +
              P.R. of China<br />
 +
              Zip: 210046
 +
            </p>
 +
            <p><strong><u>Email</u></strong></p>
 +
            <p>nanjing_china@163.com</p>
 +
            <p><strong><u>Social media</u></strong></p>
 +
            <p>Twitter button: <br/>
 +
            <a href="https://twitter.com/Guja1501">iGEM Nanjing-China@nanjing_china</a></p>
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        </div>
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        <div class="f-img">
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          <div align="center"><img src="https://static.igem.org/mediawiki/2017/0/04/T-Nanjing-China-%E5%8D%97%E4%BA%AC%E5%A4%A7%E5%AD%A6.png" name="img1" width="160" height="220" id="img1" onmouseover="MM_changeProp('img1','','width','180px','IMG');MM_changeProp('img1','','height','240px','IMG')" onmouseout="MM_changeProp('img1','','width','160px','IMG');MM_changeProp('img1','','height','220px','IMG')" /></div>
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        </div>
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          <div class="f-img">
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            <div align="right"><img src=" https://static.igem.org/mediawiki/2017/d/d4/T-Nanjing-China-%E5%9B%BE%E7%89%873.png
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          </div>
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          <div class="f-img">
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            <div align="center"><img src="https://static.igem.org/mediawiki/2017/3/37/T-Nanjing-China-s1.png" name="img3" width="160" height="160" id="img3"  onmouseover="MM_changeProp('img3','','width','180px','IMG');MM_changeProp('img3','','height','180px','IMG')" onmouseout="MM_changeProp('img3','','width','160px','IMG');MM_changeProp('img3','','height','160px','IMG')" align="absmiddle" /></div>
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          </div>
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          <div class="f-img">
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            <div align="right"><img src="https://static.igem.org/mediawiki/2017/d/dc/T-Nanjing-China-%E5%9B%BE%E7%89%872.png" name="img4" width="160" height="160" id="img4"  onmouseover="MM_changeProp('img4','','width','180px','IMG');MM_changeProp('img4','','height','180px','IMG')" onmouseout="MM_changeProp('img4','','width','160px','IMG');MM_changeProp('img4','','height','160px','IMG')" /></div>
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        </div>
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      <div class="f-b"><img src="https://static.igem.org/mediawiki/2017/9/91/T-Nanjing-China-foot.png" width="100%" /></div>
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Revision as of 07:37, 26 October 2017

Team:Nanjing-China - 2017.igem.org

H2: BBa_K2257000

SQR is a NAD(P)/FAD-dependent oxidoreductase, which oxidizes S2- to zero-valent S0. SqrR, encoded by sqrR, is a constitutively expressed suppressor protein of sqr promotor. RFP is the indicator. When there is no H2S in the environment, SqrR combines with the sqr promotor and keeps the expression of both the enzyme and RFP at a low level. While in the case where H2S exists, it will be oxidized by the small amount of expressed enzyme, and the product will interact with SqrR, forming a tri- or tetrasulfide cross-links linking C41 and C107 on the same subunit of SqrR. The change in the structure of SqrR makes it fall off from the sqr promotor and thus the expression of SQR and RFP turns on. The part can be used to detect hydrogen sulfide at a μmol level.

H2S: BBa_K2257006

SQR is a NAD(P)/FAD-dependent oxidoreductase, which oxidizes S2- to zero-valent S0. SqrR, encoded by sqrR, is a constitutively expressed suppressor protein of sqr promotor. RFP is the indicator. When there is no H2S in the environment, SqrR combines with the sqr promotor and keeps the expression of both the enzyme and RFP at a low level. While in the case where H2S exists, it will be oxidized by the small amount of expressed enzyme, and the product will interact with SqrR, forming a tri- or tetrasulfide cross-links linking C41 and C107 on the same subunit of SqrR. The change in the structure of SqrR makes it fall off from the sqr promotor and thus the expression of SQR and RFP turns on. The part can be used to detect hydrogen sulfide at a μmol level.

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china