Difference between revisions of "Team:SiCAU-China"

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<h1 align="center" class="STYLE6">A Positive Feedback System to Detection</h1>
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<h1 align="center" class="STYLE6">A Sensitive Positive Feedback Detector</h1>
 
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Antibiotic is one of the most popular drug in the world, it can be used in medical treatment, agriculture, food processing, research and so on. But overusing antibiotics may bring antibiotics residue to environment, which will cause potential damage to human and impact the ecotope. Nowadays there are some physical and chemical methods to detect antibiotics residue, but they often need high cost and complicated process. So this year our team focus on Biosensor, said to be a easier way to detect than traditional methods at a lower price, to realize the trace detection.<br />
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Positive feedback system can be found in everywhere, existed widely in creature contained from Lac operon of prokaryotes to human beings, and it has been used to construct various attractive circuits such bistable state, oscillators and other inconceivable systems. In our project this year, the significant performance of sensitivity, which researchers has not concerned to, will be developed in positive feedback loop established by LuxR/LuxI quorum sensing system. But this loop will be open because of its background expression. The AiiA, which can hydrolyze the AHL, will be control the background expression, together with the Lac operon being the system main switch. Furthermore, the input defined by user will have a negative correlation to the opening time. Hence, there need a model to describe this relation and even realize quantitative determination.
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The positive feedback loop has been widely applied in synthetic biology, such as logic gates, oscillators, bistable circuit. Besides, the positive feedback loop is also an ultra-sensitive circuit, just like Polymerase Chain Reaction (PCR), even be used in quantitative expression detected. So we engineered a biosensor, which will be established a positive feedback loop using luxI/luxR system in E.coli, to achieve trace detection. Similar to the detect theory of real-time quantitative PCR, there will be a relation between the content of input signal and the time to open the loop. We will use this system to do some antibiotics leftover detections.
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<p style="font-size:22px;color:#CCCC66;width: 94%;margin: 0 auto;">more coming soon ......</p>
 
<p style="font-size:22px;color:#CCCC66;width: 94%;margin: 0 auto;">more coming soon ......</p>

Revision as of 07:07, 2 September 2017

A Sensitive Positive Feedback Detector

Positive feedback system can be found in everywhere, existed widely in creature contained from Lac operon of prokaryotes to human beings, and it has been used to construct various attractive circuits such bistable state, oscillators and other inconceivable systems. In our project this year, the significant performance of sensitivity, which researchers has not concerned to, will be developed in positive feedback loop established by LuxR/LuxI quorum sensing system. But this loop will be open because of its background expression. The AiiA, which can hydrolyze the AHL, will be control the background expression, together with the Lac operon being the system main switch. Furthermore, the input defined by user will have a negative correlation to the opening time. Hence, there need a model to describe this relation and even realize quantitative determination.

more coming soon ......