Difference between revisions of "Team:Queens Canada/Results"

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<h1>Results</h1>
 
<h1>Results</h1>
  
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<p>Here you can describe the results of your project and your future plans. </p>
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<p>MhLap Dextran Binding</p>
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<p>The first step in assessing the feasibility of using dextran to crosslink M. hydrocarbonoclasticus to our biofilm was to test the ability of this bacterium’s sugar binding domain to bind dextran. Luckily, Tyler Vance of Dr. Davies’ lab already had the sugar-binding MhLap Region III cloned into pET-28 for overexpression in E. coli. We expressed, then purified this construct using a Ni-NTA column. Purified MhLap Region III fractions were pooled.
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We then set up a simple affinity chromatography binding assay. A Pasteur pipette was filled half-way with sephadex G-200 slurry. Purified MhLap was then added, followed by five washes with buffer. We then washed twice with 5 mg/mL dextran solution. Finally, the column was washed twice with EDTA to nonspecifically elute all protein. The collected fractions can be seen in the following SDS gels:</p>
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<h5>What should this page contain?</h5>
 
<h5>What should this page contain?</h5>
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<h5>You should also describe what your results mean: </h5>
 
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
 
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
 
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
 
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<div class="clear"></div>
 
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<h5> Project Achievements </h5>
 
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
 
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<ul>
 
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<li>A list of linked bullet points of the successful results during your project</li>
 
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
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<h5>Inspiration</h5>
 
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<p>See how other teams presented their results.</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Revision as of 19:02, 28 August 2017

Results

MhLap Dextran Binding

The first step in assessing the feasibility of using dextran to crosslink M. hydrocarbonoclasticus to our biofilm was to test the ability of this bacterium’s sugar binding domain to bind dextran. Luckily, Tyler Vance of Dr. Davies’ lab already had the sugar-binding MhLap Region III cloned into pET-28 for overexpression in E. coli. We expressed, then purified this construct using a Ni-NTA column. Purified MhLap Region III fractions were pooled. We then set up a simple affinity chromatography binding assay. A Pasteur pipette was filled half-way with sephadex G-200 slurry. Purified MhLap was then added, followed by five washes with buffer. We then washed twice with 5 mg/mL dextran solution. Finally, the column was washed twice with EDTA to nonspecifically elute all protein. The collected fractions can be seen in the following SDS gels:

What should this page contain?
  • Clearly and objectively describe the results of your work.
  • Future plans for the project.
  • Considerations for replicating the experiments.