Difference between revisions of "Team:UCSC/Model"

Line 33: Line 33:
 
         </br>
 
         </br>
 
         </div>
 
         </div>
         $$\frac{0.774\ mmol\ FWY}{1g\  protein}\times \frac{0.6g\  protein}{1g\  biomass} \approx \frac{0.46\ mmol \ chor.}{1g\ biomass}\rightarrow\frac{1\ mol\ acet.}{4\ mol\ chor.}\times\frac{151.163g}{1\ mol\ acet.}=\frac{17.56g\ acet.}{1g\ biomass}$$
+
         $$\frac{0.6g\  protein}{1g\  biomass} \approx \frac{0.46\ mmol \ chor.}{1g\ biomass}\rightarrow\frac{1\ mol\ acet.}{4\ mol\ chor.}\times\frac{151.163g}{1\ mol\ acet.}=\frac{17.56g\ acet.}{1g\ biomass}$$
 
         (Equation producing acetaminophen)
 
         (Equation producing acetaminophen)
 
     </div>
 
     </div>
Line 68: Line 68:
 
         <h3>References</h3>
 
         <h3>References</h3>
 
         <a href= http://onlinelibrary.wiley.com/doi/10.1002/cjce.22154/abstract>Growth optimization of Synechococcus elongatus PCC7942 in lab flasks and a 2-D photobioreactor</a> </br>
 
         <a href= http://onlinelibrary.wiley.com/doi/10.1002/cjce.22154/abstract>Growth optimization of Synechococcus elongatus PCC7942 in lab flasks and a 2-D photobioreactor</a> </br>
         <a href= http://ucelinks.cdlib.org:8888/sfx_local?ID=doi:10.1007%2Fs10811-011-9683-2&genre=article&atitle=Carbon%20metabolism%20and%20energy%20conversion%>Carbon metabolism and energy conversion of Synechococcus sp. PCC 7942 under mixotrophic conditions.</a>
+
         <a href= http://ucelinks.cdlib.org:8888/sfx_local?ID=doi:10.1007%2Fs10811-011-9683-2&genre=article&atitle=Carbon%20metabolism%20and%20energy%20conversion%>Carbon metabolism and energy conversion of Synechococcus sp. PCC 7942 under mixotrophic conditions.</a></br>
         <a href=https://ndb.nal.usda.gov/ndb/foods/show/3306?fgcd=&manu=&lfacet=&format=Full&count=&max=50&offset=&sort=default&order=asc&qlookup=11667&ds=&qt=&qp=&qa=&qn=&q=&ing=>United States Department of Agriculture Full Nutritional Report, Spirulina</a>
+
         <a href=https://ndb.nal.usda.gov/ndb/foods/show/3306?fgcd=&manu=&lfacet=&format=Full&count=&max=50&offset=&sort=default&order=asc&qlookup=11667&ds=&qt=&qp=&qa=&qn=&q=&ing=>United States Department of Agriculture Full Nutritional Report, Spirulina</a></br>
         <a href=http://onlinelibrary.wiley.com/store/10.1002/jsfa.2740330511/asset/2740330511_ftp.pdf?v=1&t=j7tly39i&s=d889996cde56001a928eced82e3506459f823956>Nutritional Quality of the Blue-Green Alga
+
         <a href=http://onlinelibrary.wiley.com/store/10.1002/jsfa.2740330511/asset/2740330511_ftp.pdf?v=1&t=j7tly39i&s=d889996cde56001a928eced82e3506459f823956>Nutritional Quality of the Blue-Green Alga Spirulina platensis. </a>
Spirulina platensis. </a>
+
 
     </div>
 
     </div>
 
     </body>
 
     </body>

Revision as of 22:51, 20 September 2017

Modeling

Predict and optimize yield.

Acetaminophen

To predict theoretical acetaminophen production, we calculated the amount of its precursor, chorismate, by quantifying its main products, the aromatic amino acids phenylalanine, tyrosine, and tryptophan. Since no amino acid composition data was available for Synechococcus, we started by using literature data for the similar cyanobacteria species Spirulina found that between 11 and 13.6 percent of amino acids were aromatics by mass, or between 6.5 and 7.7 molar percent of total protein. To further verify our organism's amount of acetaminophen precursor, we ran both the genome and ribosomal protein sequences through a custom Python program converting codons to amino acids and calculating aromatic amino acid molar percentages which resulted in 9.3% and 5.14% respectively.
$$\frac{0.774\ mmol\ FWY}{1g\ protein}\times \frac{0.6g\ protein}{1g\ biomass} \approx \frac{0.46\ mmol \ chor.}{1g\ biomass}\rightarrow\frac{1\ mol\ acet.}{4\ mol\ chor.}\times\frac{151.163g}{1\ mol\ acet.}=\frac{17.56g\ acet.}{1g\ biomass}$$ (Equation/ table summing amino acids converting to molar percentage)
Using our sequence generated median chorismate value of 9.3% and the assumption that our enzymes would take a third of the chorismate precursor, we got an estimate for acetaminophen concentration of between 17.5mg and 1.29 mg per gram dried biomass. These numbers show that there is likely enough precursor and that acetaminophen production should be within a measurable range.
$$\frac{0.6g\ protein}{1g\ biomass} \approx \frac{0.46\ mmol \ chor.}{1g\ biomass}\rightarrow\frac{1\ mol\ acet.}{4\ mol\ chor.}\times\frac{151.163g}{1\ mol\ acet.}=\frac{17.56g\ acet.}{1g\ biomass}$$ (Equation producing acetaminophen)

Biomass

To understand the production capacity of our organism, we aggregated growth data from published papers and all of our lab’s growth data. Using limited logistic growth curves and linear algebra to fit our equation, we modelled dried biomass and cell count per time, with the additional dependent variables of temperature, light intensity, and starter culture density.
Timescale: days
Light Intensity: μE m-2 s-1
Temperature:
Starting Density: g biomass/ L

References

Growth optimization of Synechococcus elongatus PCC7942 in lab flasks and a 2-D photobioreactor
Carbon metabolism and energy conversion of Synechococcus sp. PCC 7942 under mixotrophic conditions.
United States Department of Agriculture Full Nutritional Report, Spirulina
Nutritional Quality of the Blue-Green Alga Spirulina platensis.
Jack Baskin School of Engineering
University of California, Santa Cruz
1156 High Street
Santa Cruz, California, 95064
Contact Us: UCSC iGEM