Difference between revisions of "Team:Hong Kong UCCKE/Notebook"

Line 158: Line 158:
 
<div class="divider"></div>
 
<div class="divider"></div>
 
<p style="text-align:left !important;">7th iGEM Meeting <br>
 
<p style="text-align:left !important;">7th iGEM Meeting <br>
−
Location: Lab
+
Location: Lab<br>
−
Date: 7/3/2017
+
Date: 7/3/2017<br>
−
Time: 16:15-17:45
+
Time: 16:15-17:45<br>
−
Attendance:All  of the members  
+
Attendance:All  of the members <br>
 
We had a regular meeting today.
 
We had a regular meeting today.
 
</p>
 
</p>
Line 177: Line 177:
 
<div class="weekcontent week17content">
 
<div class="weekcontent week17content">
 
<div class="divider"></div>
 
<div class="divider"></div>
−
<p style="text-align:left !important;">8th iGEM Meeting
+
<p style="text-align:left !important;">8th iGEM Meeting<br>
−
Location: Lab
+
Location: Lab<br>
−
Date: 20/3/2017
+
Date: 20/3/2017<br>
−
Time: 16:00-17:30
+
Time: 16:00-17:30<br>
−
Attendance: All of the members
+
Attendance: All of the members<br>
−
Decided our research details
+
Decided our research details, Human Practice, Collaboration details and division of labour<br>
−
Decided HP, Collab details
+
−
Work division
+
  
 
<p>
 
<p>
−
9th iGEM Meeting  
+
9th iGEM Meeting <br>
−
Location: PMQ @ 35 Aberdeen Street, Central, Hong Kong  
+
Location: PMQ @ 35 Aberdeen Street, Central, Hong Kong <br>
−
Date: 22/3/2017
+
Date: 22/3/2017<br>
−
Time: 19:00-21:30
+
Time: 19:00-21:30<br>
−
Attendance: All of the members  
+
Attendance: All of the members <br>
−
We attended a talk organized by DIY Bio, a community lab in Hong Kong featuring Genome Editing. The talk was given by Dr Alan S.L. Wong, an assistant professor of the School of the Biomedical Sciences of the University of Hong Kong. We established a collaborative relationship with the community lab and had received many pieces of precious advice from the seniors from the field in particular.  
+
We attended a talk organized by DIY Bio, a community lab in Hong Kong featuring Genome Editing. <br>
 +
The talk was given by Dr Alan S.L. Wong, an assistant professor of the School of the Biomedical Sciences<br>
 +
of the University of Hong Kong. We established a collaborative relationship with the community lab <br>
 +
and had received many pieces of precious advice from the seniors from the field in particular.  
 
</p>
 
</p>
 
</div>
 
</div>
Line 200: Line 201:
 
<div class="weekcontent week18content">
 
<div class="weekcontent week18content">
 
<div class="divider"></div>
 
<div class="divider"></div>
−
<p style="text-align:left !important;">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</p>
+
<p style="text-align:left !important;">10th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 27/3/2017<br>
 +
Time: 16:00-17:30<br>
 +
Attendance: Most of the members<br>
 +
We had a meeting with four guest teachers from G. T. (Ellen Yeung) College. Sharing our experience of <br>
 +
participating in the 2016 iGEM, we hope to help them with their establishment of their new iGEM team.  
 +
 
 +
<p>
 +
11th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 30/3/2017<br>
 +
Time: 16:00-17:30<br>
 +
Attendance: Most of the members <br>
 +
We listed most pathways that produces uric acid, did some digging on those pathways, trying to come up <br>
 +
with a method to regulate the production of uric acid and we comb our data by writing the information we <br>
 +
have on hand on the windows in lab!
 +
</p>
 
</div>
 
</div>
  
Line 207: Line 225:
 
<div class="weekcontent week19content">
 
<div class="weekcontent week19content">
 
<div class="divider"></div>
 
<div class="divider"></div>
−
<p style="text-align:left !important;">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</p>
+
<p style="text-align:left !important;">12th iGEM Meeting <br>
 +
Location: HKUST<br>
 +
Date: 3/4/2017-4/4/2017<br>
 +
Time: 09:30-17:30 <br>
 +
Attendance: All members <br>
 +
We attended another workshop called Regulatory issues associated with Genome Editing and Gene Drive - <br>
 +
Regulation of Synthetic Biology in Asia organized by professor K. L. Chow and his team from the Hong Kong <br>
 +
University of Science and Technology. Bioethics and more information about the latest genome editing technology, <br>
 +
CRISPR is given. We established collaborative relationships with HKUST and CUHK 2017 iGEM team respectively. <br>
 +
It was a fruitful workshop.
 +
</p>
 
</div>
 
</div>
  
Line 242: Line 270:
 
<div class="week content week24content">
 
<div class="week content week24content">
 
<div class="divider"></div>
 
<div class="divider"></div>
−
<p style="text-align:left !important;">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</p>
+
<p style="text-align:left !important;">13th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 8/5/2017<br>
 +
Time: 16:00-17:30<br>
 +
Attendance: Most of the members <br>
 +
We continued learning how to design parts and order it.
 +
 
 +
</p>
 
</div>
 
</div>
  
Line 263: Line 298:
 
<div class="weekcontent week27content">
 
<div class="weekcontent week27content">
 
<div class="divider"></div>
 
<div class="divider"></div>
−
<p style="text-align:left !important;">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</p>
+
<p style="text-align:left !important;">14th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 30/5/2017<br>
 +
Time: 15:20-17:30<br>
 +
Attendance: Most of the members <br>
 +
We have finalized the design of 3 parts.  
 +
</p>
 
</div>
 
</div>
  
Line 291: Line 332:
 
<div class="weekcontent week31content">
 
<div class="weekcontent week31content">
 
<div class="divider"></div>
 
<div class="divider"></div>
−
<p style="text-align:left !important;">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</p>
+
<p style="text-align:left !important;">15th iGEM Meeting <br>
 +
Location: Lab <br>
 +
Date 29/6/2017<br>
 +
Time: 13:15-15:00<br>
 +
Attendance: Most of our members <br>
 +
We had a short meeting after our exams to finalize our project progress and deadlines. <br>
 +
We are also briefed the guideline of the projects from iGEM
 +
</p>
 
</div>
 
</div>
  
Line 298: Line 346:
 
<div class="weekcontent week20content">
 
<div class="weekcontent week20content">
 
<div class="divider"></div>
 
<div class="divider"></div>
−
<p style="text-align:left !important;">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</p>
+
<p style="text-align:left !important;">16th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 4/7/2017<br>
 +
Time: 10:30-12:30 <br>
 +
Attendance: Most of the members <br>
 +
Attendees were informed the blueprint of our wet lab schedule, they grasped an understanding on <br>
 +
the whole process of our phase 1 project. Protocols of the corresponding procedures were also combed.
 +
 
 +
<p>
 +
17th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 5/7/2017<br>
 +
Time: 09:00-17:30<br>
 +
Attendance: Most of the members <br>
 +
Our phase 1 wet lab schedule was finalized and pre-lab calculations were done. New iGEMers had a bit <br>
 +
hands on wet lab training as well as presentation training. They tried to present our project once to the old iGEMers. <br>
 +
Throughout the process, they were taught some presentation skills and some conceptual mistakes were corrected.
 +
</p>
 
</div>
 
</div>
  
Line 305: Line 370:
 
<div class="weekcontent week33content">
 
<div class="weekcontent week33content">
 
<div class="divider"></div>
 
<div class="divider"></div>
−
<p style="text-align:left !important;">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</p>
+
<p style="text-align:left !important;">18th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 11/7/2017<br>
 +
Time: 15:00-17:00<br>
 +
Attendance: Most of the members <br>
 +
We prepared agar plate and SOB medium. Cells were seeded as well <br>
 +
 
 +
<p>
 +
19th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 12/7/2017<br>
 +
Time: 09:00-17:00<br>
 +
Attendance: Most of the members <br>
 +
We prepared competent cells, did restriction enzyme digestion, gel purification, ligation and transformation <br>
 +
 
 +
<p>
 +
20th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 13/7/2017<br>
 +
Time: 15:30-17:30<br>
 +
Attendance: Most of the members <br>
 +
We cloned our cells and did liquid medium culture<br>
 +
 
 +
<p>
 +
21st iGEM Meeting <br>
 +
Location: Lab <br>
 +
Date: 14/7/2017<br>
 +
Time: 10:00-12:00<br>
 +
Attendance: Most of the members <br>
 +
We did miniprep and stored cells for further experiments. <br>
 +
</p>
 
</div>
 
</div>
  
Line 312: Line 407:
 
<div class="weekcontent week34content">
 
<div class="weekcontent week34content">
 
<div class="divider"></div>
 
<div class="divider"></div>
−
<p style="text-align:left !important;">Lorem ipsum dolor sit amet, consectetur adipiscing elit.</p>
+
<p style="text-align:left !important;">22nd iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 18/7/2017<br>
 +
Time: 14:00-16:30<br>
 +
Attendance: Some of the members <br>
 +
We prepared SOB medium and trained new iGEMers’ presentation skills. <br>
 +
Liquid culture and cell passing were also performed
 +
 
 +
<p>
 +
23rd iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 19/7/2017<br>
 +
Time: 10:00-12:30<br>
 +
Attendance: Some of the members <br>
 +
We did miniprep and gel electrophoresis of cloone checking. Training on presentation skills was done. <br>
 +
 
 +
<p>
 +
24th iGEM Meeting <br>
 +
Location: Lab<br>
 +
Date: 22/7/2017<br>
 +
Time: 10:00-12:30<br>
 +
Attendance: Most of the members<br>
 +
We organized the second meeting with iGEM 2017 Team CUHK to further discuss collaboration details. <br>
 +
We reached a consensus regarding the date and time borrowing their laboratory for our experiment as our <br>
 +
school laboratory is less well-equipped comparing to theirs. In return, we helped them build their ideal model <br>
 +
using 3D printing design software. We all had a fruitful meeting.
 +
</p>
 
</div>
 
</div>
  

Revision as of 14:14, 13 October 2017

Notebook

Week 1 (1-3/12/2016)

Oops... we haven't started preparing... consider start searching from Week 9?

Week 2 (4-10/12/2016)

Oops... we haven't started preparing... consider start searching from Week 9?

Week 3 (11-17/12/2016)

Oops... we haven't started preparing... consider start searching from Week 9?

Week 4 (18-24/12/2016)

Oops... we haven't started preparing... consider start searching from Week 9?

Week 5 (25-31/12/2016)

Oops... we haven't started preparing... consider start searching from Week 9?

Week 6 (1-7/1/2017)

Oops... we haven't started preparing... consider start searching from Week 9?

Week 7 (8-14/1/2017)

Oops... we haven't started preparing... consider start searching from Week 9?

Week 8 (15-21/1/2017)

Oops... we haven't started preparing... consider start searching from Week 9?

Week 9 (22-28/1/2017)

2nd iGEM Meeting
Location: Lab
Date: 23/1/2017
Time: 16:15-17:45
Attendance: All helpers
In today’s meeting, we taught new members some basic knowledge about genetic engineering,
including promoter, terminator, RBS etc.. All members were assigned to design at least one part
as homework and contribution to the team

Week 10 (29/1/2017-4/2/2017)

Uh oh... we were lazy this week... wanna see what's happening next week?

Week 11 (5-11/2/2017)

3rd iGEM Meeting
Location: Lab
Date: 6/2/2017
Time: 16:15-18:00
Attendance: Most of the members
In today’s meeting, we taught new members how to do gel electrophoresis.
They were taught the purpose of doing gel electrophoresis and they gave a try on doing it.
Core members in the team had meeting allocating manpower and set a solid schedule.

Week 12 (12-18/1/2017)

4th iGEM Meeting
Location: Lab
Date: 13/2/2017
Time: 16:15-17:50
Attendance: Most of the members
We had a regular meeting today.

Week 13 (19-25/1/2017)

5th iGEM Meeting
Location: Lab
Date: 20/2/2017
Time: 16:15-17:50
Attendance: All core members
In today’s meeting, we reported our research progress and started assembling our project.
We have also done an unofficial interview to the potential new iGEMers.

Week 14 (26/2/2017-4/3/2017)

6th iGEM Meeting
Location: Lab
Date: 27/2/2017
Time: 16:15-18:00
Attendance: Most of the members
In today’s meeting, 7th graders showed us their STEM (Science, Technology, Engineering and Mathematics) project.
We were the judges in their competition. After the competition, core members of the team stayed behind to
discuss the further details of our iGEM trip.

Week 15 (5-11/3/2017)

7th iGEM Meeting
Location: Lab
Date: 7/3/2017
Time: 16:15-17:45
Attendance:All of the members
We had a regular meeting today.

Week 16 (12-18/3/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 17 (19-25/3/2017)

8th iGEM Meeting
Location: Lab
Date: 20/3/2017
Time: 16:00-17:30
Attendance: All of the members
Decided our research details, Human Practice, Collaboration details and division of labour

9th iGEM Meeting
Location: PMQ @ 35 Aberdeen Street, Central, Hong Kong
Date: 22/3/2017
Time: 19:00-21:30
Attendance: All of the members
We attended a talk organized by DIY Bio, a community lab in Hong Kong featuring Genome Editing.
The talk was given by Dr Alan S.L. Wong, an assistant professor of the School of the Biomedical Sciences
of the University of Hong Kong. We established a collaborative relationship with the community lab
and had received many pieces of precious advice from the seniors from the field in particular.

Week 18 (26/3/2017-1/4/2017)

10th iGEM Meeting
Location: Lab
Date: 27/3/2017
Time: 16:00-17:30
Attendance: Most of the members
We had a meeting with four guest teachers from G. T. (Ellen Yeung) College. Sharing our experience of
participating in the 2016 iGEM, we hope to help them with their establishment of their new iGEM team.

11th iGEM Meeting
Location: Lab
Date: 30/3/2017
Time: 16:00-17:30
Attendance: Most of the members
We listed most pathways that produces uric acid, did some digging on those pathways, trying to come up
with a method to regulate the production of uric acid and we comb our data by writing the information we
have on hand on the windows in lab!

Week 19 (2-8/4/2017)

12th iGEM Meeting
Location: HKUST
Date: 3/4/2017-4/4/2017
Time: 09:30-17:30
Attendance: All members
We attended another workshop called Regulatory issues associated with Genome Editing and Gene Drive -
Regulation of Synthetic Biology in Asia organized by professor K. L. Chow and his team from the Hong Kong
University of Science and Technology. Bioethics and more information about the latest genome editing technology,
CRISPR is given. We established collaborative relationships with HKUST and CUHK 2017 iGEM team respectively.
It was a fruitful workshop.

Week 20 (9-15/4/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 21 (16-22/3/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 22 (23-29/4/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 23 (30/4/2017-6/5/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 24 (7-13/5/2017)

13th iGEM Meeting
Location: Lab
Date: 8/5/2017
Time: 16:00-17:30
Attendance: Most of the members
We continued learning how to design parts and order it.

Week 25 (14-20/5/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 26 (21-27/5/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 27 (28/5/2017-3/6/2017)

14th iGEM Meeting
Location: Lab
Date: 30/5/2017
Time: 15:20-17:30
Attendance: Most of the members
We have finalized the design of 3 parts.

Week 28 (4-10/6/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 29 (11-17/6/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 30 (18-24/6/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 31 (25/6/2017-1/7/2017)

15th iGEM Meeting
Location: Lab
Date 29/6/2017
Time: 13:15-15:00
Attendance: Most of our members
We had a short meeting after our exams to finalize our project progress and deadlines.
We are also briefed the guideline of the projects from iGEM

Week 32 (2-8/7/2017)

16th iGEM Meeting
Location: Lab
Date: 4/7/2017
Time: 10:30-12:30
Attendance: Most of the members
Attendees were informed the blueprint of our wet lab schedule, they grasped an understanding on
the whole process of our phase 1 project. Protocols of the corresponding procedures were also combed.

17th iGEM Meeting
Location: Lab
Date: 5/7/2017
Time: 09:00-17:30
Attendance: Most of the members
Our phase 1 wet lab schedule was finalized and pre-lab calculations were done. New iGEMers had a bit
hands on wet lab training as well as presentation training. They tried to present our project once to the old iGEMers.
Throughout the process, they were taught some presentation skills and some conceptual mistakes were corrected.

Week 33 (9-15/7/2017)

18th iGEM Meeting
Location: Lab
Date: 11/7/2017
Time: 15:00-17:00
Attendance: Most of the members
We prepared agar plate and SOB medium. Cells were seeded as well

19th iGEM Meeting
Location: Lab
Date: 12/7/2017
Time: 09:00-17:00
Attendance: Most of the members
We prepared competent cells, did restriction enzyme digestion, gel purification, ligation and transformation

20th iGEM Meeting
Location: Lab
Date: 13/7/2017
Time: 15:30-17:30
Attendance: Most of the members
We cloned our cells and did liquid medium culture

21st iGEM Meeting
Location: Lab
Date: 14/7/2017
Time: 10:00-12:00
Attendance: Most of the members
We did miniprep and stored cells for further experiments.

Week 34 (16-22/7/2017)

22nd iGEM Meeting
Location: Lab
Date: 18/7/2017
Time: 14:00-16:30
Attendance: Some of the members
We prepared SOB medium and trained new iGEMers’ presentation skills.
Liquid culture and cell passing were also performed

23rd iGEM Meeting
Location: Lab
Date: 19/7/2017
Time: 10:00-12:30
Attendance: Some of the members
We did miniprep and gel electrophoresis of cloone checking. Training on presentation skills was done.

24th iGEM Meeting
Location: Lab
Date: 22/7/2017
Time: 10:00-12:30
Attendance: Most of the members
We organized the second meeting with iGEM 2017 Team CUHK to further discuss collaboration details.
We reached a consensus regarding the date and time borrowing their laboratory for our experiment as our
school laboratory is less well-equipped comparing to theirs. In return, we helped them build their ideal model
using 3D printing design software. We all had a fruitful meeting.

Week 35 (23-29/7/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 36 (30/7/2017-5/8/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 37 (6-12/8/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 38 (13-19/8/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 39 (20-26/8/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 40 (27/8/2017-2/9/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 39 (20-26/8/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 40 (27/8/2017-2/9/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

Week 41 (3-9/9/2017)

Lorem ipsum dolor sit amet, consectetur adipiscing elit.

UCCKE iGEM 2017

Contact

Quicklinks

Social Media

UCCKE team. Designed with by Gabriel Lee.