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<li><a href="https://2017.igem.org/Team:TU_Darmstadt/project" class="active">Project</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project" class="active">Project</a></li> | ||
<div class="nav-proj-drop"> | <div class="nav-proj-drop"> | ||
− | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase">Chitin | + | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase">Chitin synthase</a></li> |
<li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitinase">Chitinase</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitinase">Chitinase</a></li> | ||
− | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_deacetylase">Chitin | + | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_deacetylase">Chitin deacetylase</a></li> |
− | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/regulation_system">Regulation | + | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/regulation_system">Regulation system</a></li> |
<li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chemistry" class="active">Chemistry</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chemistry" class="active">Chemistry</a></li> | ||
<li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/modeling">Modeling</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project/modeling">Modeling</a></li> | ||
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<div class="nav-drop"> | <div class="nav-drop"> | ||
<li><a href="https://2017.igem.org/Team:TU_Darmstadt/human_practices">Human Practices</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/human_practices">Human Practices</a></li> | ||
− | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/ | + | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/hardware">Hardware</a></li> |
<li><a href="https://2017.igem.org/Team:TU_Darmstadt/team">Team</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/team">Team</a></li> | ||
<li><a href="https://2017.igem.org/Team:TU_Darmstadt/judging">Judging</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/judging">Judging</a></li> | ||
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<div class="container"> | <div class="container"> | ||
<header class="major"> | <header class="major"> | ||
− | <h2> | + | <h2>Chemical modification of Chitosan for the use in protease-sensing wound coatings.</h2><!--BIG HEADING--> |
</header> | </header> | ||
− | <p> | + | <h3>Abstract</h3> |
+ | <p> | ||
+ | Chitosan, especially a chitosan-based hydrogel, can be modified at the amino group with succinyl anhydride and a variable Peptide with a fluorogenic substrate | ||
+ | to form a reliable system to detect proteases. In this study, we reproduce the findings of the Paper “Enzyme-Sensing Chitosan Hydrogels” by Mir Morteza Sadat Ebrahimi | ||
+ | and Holger Schönherr from the university of Siegen and use the fluorogenic substrate alanyl-alanyl-phenylalanine-7-amido-4-methylcoumarin (AAP-AMC) to detect | ||
+ | α-chymotrysin. | ||
+ | This protease is secreted by Staphylococcus aureus or Pseudomonas aeruginosa, which are examples of pathogenic bacteria that can infect wounds. | ||
+ | </p> | ||
</div> | </div> | ||
</section> | </section> | ||
<section id="two"><div class="container"> | <section id="two"><div class="container"> | ||
− | <h3> | + | <h3>Introduction</h3> |
− | <p> | + | <p> |
+ | Badly healing wounds are still a big issue in clinical medicine all over the world. Especially inflamed wounds often exhibit impaired healing properties and are prone | ||
+ | to infections of opportunistic pathogenic bacteria. On the one hand, wounds have to be screened for infections extensively, on the other hand, they have to be kept wet | ||
+ | and in an Oxygen-free atmosphere for optimal healing conditions. Thus there is an obvious contradiction between the best healing conditions and the commonly | ||
+ | used | ||
+ | infection swab test, where you have to remove the wound coating. Furthermore, current swab tests needs a few days to evaluate the presence of pathogenic bacteria, | ||
+ | but it is important to get this information as soon as possible and to start the suitable treatment. | ||
+ | Ebrahimi and Schönherr developed a quick and non invasive detection | ||
+ | method for wound infections without the necessity to remove the wound coating. The principle of the test is the modification of an amino group of chitosan with | ||
+ | succinic anhydride to gain a carboxyl group that can be linked to the amino group of our alanyl-alanyl-phenylalanine peptide linker. | ||
+ | This peptide linker is fused to | ||
+ | our actual detectable unit, the methylcoumarin.</p> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/4/4a/T--TU_Darmstadt--Flourophor_linken.jpg" /> | ||
+ | <p>This linker was chosen due to the fact that chymotrysin cleaves peptides n-terminal of aromatic amino acids.</p> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/25/T--TU_Darmstadt--cleavedge.jpg" /> | ||
+ | <p>In our studies, we present an uncomplicated method to repeat the work of Ebrahimi and Schönherr in a way that works for iGEMers and FabLabers. | ||
+ | For that, we combined a few instructions to guarantee a working product without the need of expensive instrumental analysis.</p> | ||
</div> | </div> | ||
</section> | </section> |
Revision as of 18:04, 13 October 2017