Difference between revisions of "Team:TP-CC San Diego/LabBook"

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<h3 class = "day"> DAY 4 </h3>
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<h3 class = "day"> DAY 9 </h3>
 
    
 
    
 
     <div class = "description">  
 
     <div class = "description">  
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         </p>
 
         <ul>
 
         <ul>
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             <li>Used same,r </li>
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             <li>Used same,remaining ligation reaction and transformed again onto carb antibiotic plates </li>
 +
        </ul>
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    </div>
 +
 
 +
<h3 class = "day"> DAY 10 </h3>
 +
 
 +
    <div class = "description">
 +
        <p>
 +
            <b>SUMMARY of August 14th: </b>
 +
        </p>
 +
        <ul>
 +
            <li>Inoculate comp cells </li>
 +
        </ul>
 +
    </div>
 +
 
 +
<h3 class = "day"> DAY 11 </h3>
 +
 
 +
    <div class = "description">
 +
        <p>
 +
            <b>SUMMARY of August 15th: </b>
 +
        </p>
 +
        <ul>
 +
            <li>Miniprep gRNA plasmids and sent in for sequencing </li>
 +
            <li>Start interlab study</li>
 +
            <li>Ludox Calibration</li>
 +
            <li>Fluorescein Dilution</li>
 +
            <li>Transform plasmids from plate 7 in kit provided by IGEM for interlab study</li>
 +
        </ul>
 +
    </div>
 +
 
 +
<h3 class = "day"> DAY 12 </h3>
 +
 
 +
    <div class = "description">
 +
        <p>
 +
            <b>SUMMARY of August 16th: </b>
 +
        </p>
 +
        <ul>
 +
            <li>Received sequencing results and all plasmids have the gRNA  </li>
 +
            <li>Inoculate plasmids for interlab study</li>
 
         </ul>
 
         </ul>
 
     </div>
 
     </div>
 
</div>
 
</div>
 +
<h3 class = "day"> DAY 13 </h3>
 +
 
 +
    <div class = "description">
 +
        <p>
 +
            <b>SUMMARY of August 17th: </b>
 +
        </p>
 +
        <ul>
 +
            <li>Interlab cell measurement </li>
 +
        </ul>
 +
    </div>
  
  

Revision as of 04:38, 15 October 2017


Lab Notebook

DAY 1

SUMMARY of July 17th:

  • DNA extraction from GBM39 cells
  • Agarose gel electrophoresis to confirm DNA

DAY 2

SUMMARY of July 18th:

  • qPCR primer design and order

DAY 3

SUMMARY of July 21:

  • Primers arrived

DAY 4

SUMMARY of August 1st:

DAY 5

SUMMARY of August 2nd:

  • Received oligos

DAY 6

SUMMARY of August 7th:

  • Digestion of lentiviral CRISPR plasmid
  • Gel purification digested plasmid using QIAquick Gel Extraction Kit and eluted in buffer EB
  • There should be two bands on the gel, one at 2kb which should not be purified. Purify only the larger band

DAY 7

SUMMARY of August 9th:

  • Anneal oligo pairs
  • Ligation and transformation

DAY 8

SUMMARY of August 10th:

  • No colonies grew on carb antibiotic plates

DAY 9

SUMMARY of August 11th:

  • Used same,remaining ligation reaction and transformed again onto carb antibiotic plates

DAY 10

SUMMARY of August 14th:

  • Inoculate comp cells

DAY 11

SUMMARY of August 15th:

  • Miniprep gRNA plasmids and sent in for sequencing
  • Start interlab study
  • Ludox Calibration
  • Fluorescein Dilution
  • Transform plasmids from plate 7 in kit provided by IGEM for interlab study

DAY 12

SUMMARY of August 16th:

  • Received sequencing results and all plasmids have the gRNA
  • Inoculate plasmids for interlab study

DAY 13

SUMMARY of August 17th:

  • Interlab cell measurement