Difference between revisions of "Team:NWU-CHINA/Notebook"

(Prototype team page)
 
Line 3: Line 3:
  
  
−
<div class="column full_size">
+
<script language="javascript">
 +
function show(id)
 +
{
 +
var div= ["child1","child2","child3","child4","child5","child6","child7","child8","child9"]
 +
for(i=0;i<9;i++)
 +
{
 +
document.getElementById(div[i]).style.display=(i==id?"block":"none")
 +
}
 +
}
 +
 +
</script>
 +
<body>
 +
<div class="main">
 +
<a href="#" onMouseOver="show(0)">
 +
      <div>March 15th- April 13th</div>
 +
    </a>
 +
      <div id="child1" style="display:none;">
 +
    <p>We noticed Igem competition and get acquainted with synthesis biology. </p>
 +
    </div>
 +
<a href="#" onMouseOver="show(1)">
 +
    April 14th-May 1st
 +
      </a>
 +
      <div id="child2" style="display:none;">
 +
      <p>Our team, NWU-CHINA, had been organized. Then we find our PI and confirmed our project. Our PI gave us a lot of advice about following experiment.</p>
 +
      </div>
 +
<br>  <a href="#" onMouseOver="show(2)">
 +
      May 3rd-May 20th
 +
      </a>
 +
      <div id="child3" style="display:none;">
 +
      <p>We accepted foundational lab training and learned necessary experiment operation. </p>
 +
      </div>
 +
<br>  <a href="#" onMouseOver="show(3)">
 +
      June 1st-June 20th
 +
      </a>
 +
    <div id="child4" style="display:none;">
 +
      <p>We cleared Interlab Study requirement, RFC and 3A assembly protocol. Meanwhile, we found a lot of reference paper to find theory supporting our experiment. </p>
 +
      </div>
 +
<br> <a href="#" onMouseOver="show(4)">
 +
      July 15th-July 26th
 +
      </a>
 +
      <div id="child5" style="display:none;">
 +
      <p>We finished our First Interlab Study</p>
 +
  </div>
 +
<br> <a href="#" onMouseOver="show(5)">
 +
      August 1st-August 31st
 +
    </a>
 +
    <div id="child6" style="display:none;">
 +
      <p>Using Pak1900 to construct RFP vector and transferring constructed vector into P.a to verify expression of RFP in P.a .The detail protocol is as follows: </p>
 +
<p>
 +
<ol>
 +
<li>We got RFP sequence from registry and cloned RFP gene by PCR. Then we separated cloned RFP</li>
 +
<li>gene by agarose gel electrophoresis and purified the gene by gel slices. </li>
 +
<li>We used pAK1900 as our vector. Then we used BamH I and Sac I to digest pAK1900 and purified gene for 5 hours.</li>
 +
<li>Then we used T4 DNA Ligase to ligated our vector and RFP gene at 4 ℃ for 24 hours</li>
 +
<li>We transferred our vector into E.coli DH5&alpha competent cell to get more vector clones. Our transferring protocol is as follows:</li>
 +
<ol type="a">
 +
<li>Mix 4ul vector and 50ul competent cells in 1.5mL centrifuge tube at 0 ℃ for 30 min. </li>
 +
<li>Hot shock for 90 seconds at 42℃. </li>
 +
<li>Keep competent cell at 0℃ for 4-5 min, then add 950&microL Soc media into the tube. </li>
 +
<li>Incubate at 37 ℃ for 2 hours shaking at 220rpm</li>
 +
<li>Centrifuge at 6000rpm for 1min. After centrifugation, 900&microL supernatant is discarded, then resuspended cells, spread the culture on plate with antibiotic 100&microg/&microL TC , incubate at 37℃ for 16 hours. </li>
 +
<li>Select several colonies, purified them on another selective plate, incubate at 37℃ for 12 hours. </li>
 +
</ol>
 +
<li>Incubate purified cultures with 5mL LB media and 5ul 5mg/&microL TC for 5 hours. </li>
 +
<li>Using Plasmid minipreparation kit to extract the vector.  </li>
 +
</ol>
 +
</p>
 +
      </div>
 +
<br> <a href="#" onMouseOver="show(6)"> 
 +
    September 1st-10th
 +
</a>
 +
<div id="child7" style="display:none;">
 +
<p>We had done our second Interlab Study. Meanwhile, we used PCR to clone GntR and AlkB2 gene. And ligated them on constructed RFP vector. Then we separated cloned gene by agarose gel electrophoresis and purified the gene by gel slices. </p>
 +
</div>
 +
<br> <a href="#" onMouseOver="show(7)">
 +
    September 11th-October 12th
 +
    </a>
 +
    <div id="child8" style="display:none;">
 +
<p><ol>
 +
<li>We used Hind I, Hind II, Sal I, Hind III and Xba I, Spe I and Sal I digest pAK1900, GntR, AlkB2  for 5 hours respectively. </li>
 +
<li>Then we used T4 DNA Ligase to ligated them at 4℃ for 24 hours. </li>
 +
<li>Then we cloned our vector in DH5&alpha competent cells. </li>
 +
<li>Sequence our gene to verify our vector. </li>
 +
</ol></p>
 +
</div>
 +
<br> <a href="#" onMouseOver="show(8)">
 +
    October 12th-17th
 +
    </a>
 +
    <div id="child9" style="display:none;">
 +
<p>We constructed our submission parts.</p>
 +
</div>
 +
</body>
  
−
<h1>Notebook</h1>
 
−
<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
 
−
 
−
</div>
 
−
<div class="clear"></div>
 
−
 
−
 
−
<div class="column half_size">
 
−
<h5>What should this page have?</h5>
 
−
<ul>
 
−
<li>Chronological notes of what your team is doing.</li>
 
−
<li> Brief descriptions of daily important events.</li>
 
−
<li>Pictures of your progress. </li>
 
−
<li>Mention who participated in what task.</li>
 
−
</ul>
 
−
 
−
</div>
 
−
 
−
<div class="column half_size">
 
−
<h5>Inspiration</h5>
 
−
<p>You can see what others teams have done to organize their notes:</p>
 
−
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
 
−
</ul>
 
−
 
−
</div>
 
 
</html>
 
</html>

Revision as of 07:06, 16 October 2017

<div>March 15th- April 13th</div> April 14th-May 1st
May 3rd-May 20th
June 1st-June 20th
July 15th-July 26th
August 1st-August 31st
September 1st-10th
September 11th-October 12th
October 12th-17th