Difference between revisions of "Team:Wageningen UR/Application/Device"

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                                                 The first working prototype included space for 3 membrane sealed sample vials, a positive control, the sample and a negative control. In order to take out the variables of the lighting conditions, background signal and observers we wanted to use cheap and simple electronics in order to read off the signal. This led to the removal of the ‘windows’ in the front and a bulkier design to accommodate the electronics. The improved computational approach to read off the signal was based on Light Depended Resistors (LDR). This component changes it resistance according to the amount of light that it receives. This resistance is measured via a simple circuit (See Figure) and processed by the arduino microprocessor. Once the button is pressed the device excites the sample vial with UV light and the emission spectrum is detected by the LDR’s. The signal of the sample is compared to the positive and negative control and the final result is displayed on an integrated OLED display on the front.  
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                                                 The first working prototype included space for 3 membrane sealed sample vials, a positive control, the sample and a negative control. In order to take out the variables of the lighting conditions, background signal and observers we wanted to use cheap and simple electronics in order to read off the signal. This led to the removal of the ‘windows’ in the front and a bulkier design to accommodate the electronics. The improved computational approach to read off the signal was based on Light Depended Resistors (LDR). This component changes its resistance according to the amount of light that it receives. This resistance is measured via a simple circuit (See Figure) and processed by the arduino microprocessor. Once the button is pressed the device excites the sample vial with UV light and the emission spectrum is detected by the LDR’s. The signal of the sample is compared to the positive and negative control and the final result is displayed on an integrated OLED display on the front.  
 
Although this is a mayor improvement over trying to detect the signal by eye, this way of detecting the light is unreliable.  
 
Although this is a mayor improvement over trying to detect the signal by eye, this way of detecting the light is unreliable.  
  

Revision as of 14:27, 19 October 2017

Device

In order to fully implement the point of care nature of our diagnostic, we developed a small battery powered device, which is easily brought into the field. The device is capable of measuring the fluorescence produced by our bacterial diagnostic system. The addition of a GPS sensor tags the location of the measurement for epidemiological analysis. By using 3D printing as well as the open source Arduino system, we were capable of producing an affordable product.

Working off the idea that we wanted a portable device quickly led us to a consumer 3D printer allowing us to do rapid prototyping. The printer we used is an Ultimaker 2+ provided to us by Ultimaker. We wanted a device that could show a fluorescent signal so we came we came up with our first designs. The fluorescent molecules produced by our system are excited by UV-LED’s with a specific wavelength. The emission spectrum is visible by the naked eye and this is how we planned to read out the signal. The membrane sealed sample vials containing our bacterial system are placed in the device from the top. Initially we started off with a device which fits one sample. However we soon realised that adding a positive and negative control makes out device more reliable. Once the button is pressed the UV-LED’s are turned on and the signal can be compared to the positive and negative control in order to determine the result. This approach was problematic since lighting conditions, background signal and observers are not a set variable. This prompted us rethink the way the device works and version 2 was on the way.

The first working prototype included space for 3 membrane sealed sample vials, a positive control, the sample and a negative control. In order to take out the variables of the lighting conditions, background signal and observers we wanted to use cheap and simple electronics in order to read off the signal. This led to the removal of the ‘windows’ in the front and a bulkier design to accommodate the electronics. The improved computational approach to read off the signal was based on Light Depended Resistors (LDR). This component changes its resistance according to the amount of light that it receives. This resistance is measured via a simple circuit (See Figure) and processed by the arduino microprocessor. Once the button is pressed the device excites the sample vial with UV light and the emission spectrum is detected by the LDR’s. The signal of the sample is compared to the positive and negative control and the final result is displayed on an integrated OLED display on the front. Although this is a mayor improvement over trying to detect the signal by eye, this way of detecting the light is unreliable.

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Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Aenean commodo ligula eget dolor. Aenean massa. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Donec quam felis, ultricies nec, pellentesque eu, pretium quis, sem. Nulla consequat massa quis enim. Donec pede justo, fringilla vel, aliquet nec, vulputate eget, arcu. In enim justo, rhoncus ut, imperdiet a, venenatis vitae, justo. Nullam dictum felis eu pede mollis pretium. Integer tincidunt. Cras dapibus. Vivamus elementum semper nisi. Aenean vulputate eleifend tellus. Aenean leo ligula, porttitor eu, consequat vitae, eleifend ac, enim. Aliquam lorem ante, dapibus in, viverra quis, feugiat a, tellus. Phasellus viverra nulla ut metus varius laoreet. Quisque rutrum. Aenean imperdiet. Etiam ultricies nisi vel augue. Curabitur ullamcorper ultricies nisi. Nam eget dui.

Final Prototype

Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Aenean commodo ligula eget dolor. Aenean massa. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Donec quam felis, ultricies nec, pellentesque eu, pretium quis, sem. Nulla consequat massa quis enim. Donec pede justo, fringilla vel, aliquet nec, vulputate eget, arcu. In enim justo, rhoncus ut, imperdiet a, venenatis vitae, justo. Nullam dictum felis eu pede mollis pretium. Integer tincidunt. Cras dapibus. Vivamus elementum semper nisi. Aenean vulputate eleifend tellus. Aenean leo ligula, porttitor eu, consequat vitae, eleifend ac, enim. Aliquam lorem ante, dapibus in, viverra quis, feugiat a, tellus. Phasellus viverra nulla ut metus varius laoreet. Quisque rutrum. Aenean imperdiet. Etiam ultricies nisi vel augue. Curabitur ullamcorper ultricies nisi. Nam eget dui.