Difference between revisions of "Team:WashU StLouis/Notebook"

Line 540: Line 540:
 
         <li> Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2 </li>
 
         <li> Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2 </li>
 
             <ul>
 
             <ul>
−
                   <li> Transformation was a success with 100 colonies. </li>
+
                   <li> Transformation was a success with 100 colonies </li>
 
                   <li> Plate was stored in 4 deg. Celsius for future use </li>
 
                   <li> Plate was stored in 4 deg. Celsius for future use </li>
 
             </ul>
 
             </ul>

Revision as of 02:43, 6 June 2017

HOME
Team
Project
Background
Description
Design
Model
Results
Demonstrate
Improve
Attributions
Citations
Wet Lab
Notebook
Experiments/Protocols
InterLab
Safety
Parts
Parts
Basic Parts
Composite Parts
Human Practices
Silver HP
Public Engagement
Awards
Hardware
Model
Judging Form

Notebook

June 2017
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

Micah, Maddie, Alex

  1. Prepared LB Media
  2. Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit
  3. Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells
  4. Prepared LB Agar
  5. Prepared overnight culture to make competent cells (MHD42)

Micah, Maddie

  1. Prepared MHD42 competent cells
  2. Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
    • Transformation was a success with 100 colonies
    • Plate was stored in 4 deg. Celsius for future use
  3. Prepared TSS Buffer

No Work!

No Work!

Micah, Maddie, Alex, Mark, Collin

  1. Prepared Chloramphenicol (CM) plates
  2. Prepared Ampicillin solution to be used for plates in the future
  3. Made more LB Agar