Difference between revisions of "Team:WashU StLouis/Notebook"

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     <p><strong> Lab Work </strong></p>
 
     <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 +
      <li> Checked competency test for MHD42 cells</li>
 +
            <ul>
 +
                <li> Test was successful! </li>
 +
                <li> <img src="https://static.igem.org/mediawiki/2017/0/0c/T--WashU_StLouis--2017-06-07_MHD42_Comp_Cells_Test_Results.jpg"/></li>
 +
            </ul>
 +
      <li> Checked competency test for DH5(alpha) </li>
 +
            <ul>
 +
                <li> Test showed that the cells were competent but had a low transformation efficiency </li>
 +
                <li> <img src="https://static.igem.org/mediawiki/2017/a/ae/T--WashU_StLouis--2017-06-07_DH5alpha_Comp_Cells_Test_Results.jpg"/> </li>
 
       <li> Prepared LB + Ampicillin (Amp) plates </li>
 
       <li> Prepared LB + Ampicillin (Amp) plates </li>
 
       <li> </li>
 
       <li> </li>

Revision as of 19:11, 7 June 2017

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Notebook

June 2017
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Micah, Maddie, Alex

Lab Work

  1. Prepared LB Media
  2. Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit
  3. Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation)
  4. Prepared LB Agar
  5. Prepared overnight culture to make competent cells (MHD42)

Outside Work / Discussion

Micah, Maddie

Lab Work

  1. Prepared MHD42 competent cells
  2. Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
    • Transformation was a success with 100 colonies
    • Plate was stored in 4 deg. Celsius for future use
  3. Prepared TSS Buffer

Outside Work / Discussion

No Work!

No Work!

Micah, Maddie, Alex, Mark, Collin

Lab Work

  1. Prepared LB + Chloramphenicol (CM) plates
  2. Prepared Ampicillin solution to be used for plates in the future
  3. Made more LB Agar

Outside Work / Discussion

Micah, Maddie, Alex, Mark, Collin

Lab Work

  1. Prepared DH5α competent cells
  2. Tested MHD42 cells for competency
  3. Tested DH5α cells for competency

Outside Work / Discussion

Lab Work

  1. Checked competency test for MHD42 cells
    • Test was successful!
  2. Checked competency test for DH5(alpha)
    • Test showed that the cells were competent but had a low transformation efficiency
    • Prepared LB + Ampicillin (Amp) plates

Outside Work / Discussion

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