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<h2 id="section-10" style="padding-top: 100px; margin-top: -50px;">Reagents</h2> | <h2 id="section-10" style="padding-top: 100px; margin-top: -50px;">Reagents</h2> | ||
<h4>1. LB medium(lysogeny broth)<br /> The recipe for 1l LB media is as follows:<br /> Tryptone 10g/L <br /> Yeast | <h4>1. LB medium(lysogeny broth)<br /> The recipe for 1l LB media is as follows:<br /> Tryptone 10g/L <br /> Yeast | ||
− | extract 5g/L <br /> NaCl 10g/L<br /> 2. 0.1mM Kanamycin<br /> MW of Kanamycin:582.58<br /> Store at -20℃<br /> | + | extract 5g/L <br /> NaCl 10g/L<br /><br /> 2. 0.1mM Kanamycin<br /> MW of Kanamycin:582.58<br /> Store at -20℃<br /> <br /> 3. LB plate<br /> The recipe for 1l LB plate is as follows:<br /> Tryptone 10g/L <br /> Yeast extract 5g/L <br |
− | /> NaCl 10g/L<br /> 15g Agar<br /> Add appropriate amount of resistance.<br /> 4. 2YT medium<br /> The recipe | + | /> NaCl 10g/L<br /> 15g Agar<br /> Add appropriate amount of resistance.<br /><br /> 4. 2YT medium<br /> The recipe |
− | for 1l LB plate is as follows:<br /> Tryptone 16g/L <br /> Yeast extract 10g/L <br /> NaCl 5g/L<br /> 5. 0.5mM | + | for 1l LB plate is as follows:<br /> Tryptone 16g/L <br /> Yeast extract 10g/L <br /> NaCl 5g/L<br /> <br />5. 0.5mM |
− | IPTG <br /> MW of IPTG:238.30<br /> Store at -20℃<br /> 6. 50mM PBS buffer,PH8.0<br /> A:0.05mol/L Na2HPO4<br | + | IPTG <br /> MW of IPTG:238.30<br /> Store at -20℃<br /><br /> 6. 50mM PBS buffer,PH8.0<br /> A:0.05mol/L Na2HPO4<br |
/> B:0.05mol/L KH2P04<br /> 137mMNaCl,2.7mMKCl,10mMNa2HPO4,2mMKH2PO4 for 1L.<br /> | /> B:0.05mol/L KH2P04<br /> 137mMNaCl,2.7mMKCl,10mMNa2HPO4,2mMKH2PO4 for 1L.<br /> | ||
Revision as of 04:10, 22 October 2017