Line 94: | Line 94: | ||
<body> | <body> | ||
<div id="container" style=" background-color: #02263E;"> | <div id="container" style=" background-color: #02263E;"> | ||
+ | <p><span style="color: #ffffff;"> </span></p> | ||
+ | <p><span style="color: #ffffff;"> </span></p> | ||
<p><span style="color: #ffffff;"> </span></p> | <p><span style="color: #ffffff;"> </span></p> | ||
<p><span style="color: #ffffff;"> </span></p> | <p><span style="color: #ffffff;"> </span></p> | ||
Line 116: | Line 118: | ||
</span></center></p> | </span></center></p> | ||
− | <h1>Methods</h1> | + | <center><h1>Methods</h1></center> |
<p><center>We were given 8 specimens to measure, termed “test devices”. These included a positive (BBa_I20270) and negative(BBa_R0040) control, and 6 different test devices (BBa_J36400, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004, BBa_J364005) . | <p><center>We were given 8 specimens to measure, termed “test devices”. These included a positive (BBa_I20270) and negative(BBa_R0040) control, and 6 different test devices (BBa_J36400, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004, BBa_J364005) . | ||
First we transformed these into DH5α-E. coli cells, then selected two resulting colonies to be prepared for measurement. The two colonies were grown overnight. | First we transformed these into DH5α-E. coli cells, then selected two resulting colonies to be prepared for measurement. The two colonies were grown overnight. |
Revision as of 07:20, 23 October 2017
INTERLAB
![](https://static.igem.org/mediawiki/2017/8/80/Unott_interlab_plate.jpeg)
Introduction
incomparable; separate groups often interpret fluorescence measurements in largely different ways, making it hard to properly compare results. To combat this, the interlab study is
performed each year, iteratively refining an optimal protocol for fluorescence measurement. Theoretically, this would be followed by anyone and yield comparably robust units of fluorescence, facilitating more meaningful comparisons of data between researchers. The study required us to measure a number of different fluorescent test devices sent to us by iGEM HQ, we followed their protocol and then shared the results we obtain. Teams around the world do the same; collectively, we provide a large set of data illustrating how similar the results were, potentially highlighting any aspects in which the protocol could be improved and, hopefully, showing that this is possible. This is quite an important issue, we felt we should participate and help in any way we could!
Methods
Results
LUDOX-S40 and Fluoroscein Reference Measurements
Standard Results
Interpretive Data