Difference between revisions of "Team:RPI Troy NY/Description"

Revision as of 16:47, 23 October 2017

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Project Description

♦Background♦

♦Design♦

In this project, we sought to increase the production of sophorolipids by taking a two-part approach. In the first part, we engineered primers and inserted these into the yeast to be able to produce either normal amounts of sophorolipids or lesser amounts of sophorolipids (gene knockout). In the second part, we extracted these sophorolipids and completed analysis on this. Protocols

We grew the yeast in two types of broth: FM2 and PM2. This is the components of each of those broths.

PM2 Ingredients	Amt.(g/L)	FM2 Ingredients	Amt.(g/L)
Glucose	200	Glucose	100
Yeast Extract	5	Yeast Extract	5
KH2PO4	1	KH2PO4	1
MgSO4	0.5	MgSO4	0.5
CaCl2	0.1	CaCl2	0.1
NaCl	0.1	NaCl	0.1
Peptone	0.7	Peptone	0.7

Experiments

Notebook

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 <center> <img src= "https://static.igem.org/mediawiki/2017/f/ff/Rpi_experimental_plan.png" width="550px" height="700px"> <center>
+<font face= "Times New Roman"> <font size ="3"> We grew the yeast in two types of broth: FM2 and PM2. This is the components of each of those broths.
 <table style= "width:80%">