Difference between revisions of "Team:Gaston Day School/Notebook"

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<thead>
 
<thead>
 
         <tr>
 
         <tr>
             <th>Step</th>
+
             <th>???</th>
             <th>Temperature (°C)</th>
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             <th>???</th>
             <th>Time</th>
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             <th>???</th>
 
            
 
            
 
         </tr>
 
         </tr>
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     <tbody>
 
     <tbody>
 
         <tr>
 
         <tr>
             <td>Initial denaturation</td>
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             <td>14.3</td>
             <td>98</td>
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             <td>H2O</td>
             <td>30 seconds</td>
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             <td>114.4</td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
             <td>25-35 cycles</td>
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             <td>2.5</td>
 
             <td>98 (denaturation)<br>
 
             <td>98 (denaturation)<br>
 
                 45-72 (annealing) <a href="#Note1">see Note 1</a><br>
 
                 45-72 (annealing) <a href="#Note1">see Note 1</a><br>
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     </tbody>
 
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<p id="Note1">Note 1: Use the NEB Tm calculator should be used to determine the annealing temperature when using Phusion: <a target="_blank" href="http://tmcalculator.neb.com/#!/">http://tmcalculator.neb.com/#!/</a></p>
 
 
 
</p>
 
  
 
   </div>
 
   </div>

Revision as of 04:23, 24 October 2017

Notebook

On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.

??? ??? ???
14.3 H2O 114.4
2.5 98 (denaturation)
45-72 (annealing) see Note 1
72 (extension)
5-10 seconds
10-30 seconds
15-30 seconds per kb
Final extension 72 2-5 minutes
Hold 4 Indefinitely