Difference between revisions of "Team:MSU-Michigan/IPTG Induction"
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Revision as of 20:47, 24 October 2017
IPTG Induction
M5 Media Fluorescence
Control (M5 Media only) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.
mtrB Induction Fluorescence
Induction of mtrB gene (no GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. - Acts as a second control
mtrB_GFP Induction Fluorescence
Induction of mtrB_GFP strain (GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. This is the highest because resources are not being directed toward the mtrB pathway, which is still removed
mrtB_GFP_mtrB Induction Fluorescence
Induction of mtrB_GFP_mtrB strain (GFP, mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.
Induction of mtrB GFP measured using a potentiostat under anaerobic conditions in a single cell bioreactor. The sample was induced with 200uM IPTG at 1.98 hours.
